Rabbit polyclonal antibody against native porcine collagen type I + III (heterotypic blend), affinity-purified on immobilised porcine collagen I+III and cross-absorbed against other porcine collagens and porcine plasma proteins, 1 mL. Dual-specificity (balanced anti-I + anti-III) for the native I/III heterotypic-fibril readout in porcine tissue — large-animal translational cardiovascular research (porcine MI, transplant, atherosclerosis), porcine wound-healing pharmacology, and porcine collagen-biomaterial / decellularised-ECM scaffold QC. Use cat. 223 (porcine collagen I+III antigen) as the ELISA standard, and QC porcine collagen biomaterials such as cat. 130 / cat. 785 (porcine atelocollagen 3D-gel) and cat. 144 (collagen bioink). Optimised for native-conformation work (ELISA, frozen-section IHC) — not suitable for denaturing conditions.
SPECIFICATIONS
Catalogue number: 386 | Host: Rabbit | Target: Pig (Sus scrofa)
Target antigen: Native porcine collagen type I + type III (heterotypic blend)
Gene: COL1A1 + COL3A1
Clonality: Polyclonal — dual-specificity (balanced anti-I + anti-III titres) | Conjugate: Unconjugated
Specificity: Binds native porcine collagen I + III; cross-absorbed against other porcine collagens and porcine plasma proteins. Native-conformation — not suitable for denaturing conditions.
Purification: Affinity-purified on immobilised porcine collagen I + III, with cross-absorption against porcine plasma proteins.
Format: Lyophilised, standard YO formulation | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — total-fibrillar-collagen detection in porcine tissue lysate / biomaterial extract. Pair with cat. 223 (porcine collagen I+III antigen) on the immobilised arm.
- IHC (frozen sections) — porcine MI / cardiac infarct scar, porcine wound-healing skin, porcine dermal scar.
- Cell culture / ECM staining — immunostaining of ECM in cell culture and decellularised-ECM scaffold characterisation.
- Biomaterial QC — porcine collagen biomaterials: cat. 130 / cat. 785 (porcine atelocollagen 3D-gel), cat. 144 (collagen bioink).
- Note: native-conformation antibody — not suitable for denatured Western blot.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Large-animal translational cardiovascular research — porcine MI, transplant rejection, atherosclerosis.
- Porcine wound-healing pharmacology — the gold-standard pre-clinical large-animal skin model.
- Porcine collagen-based bioink / 3D-printing material characterisation (cat. 144 collagen bioink) — ECM QC.
- Porcine decellularised-ECM scaffold quantification — xenograft scaffolds for cartilage, cardiac and dermal tissue engineering.
- Porcine atelocollagen 3D-gel ECM analysis (cat. 130, cat. 785).
