Rabbit polyclonal antibody against native human collagen type II (COL2A1, the principal cartilage collagen), affinity-purified on immobilised human collagen II and cross-absorbed against other human collagens, 0.5 mL. Binds native 3-D (triple-helical) epitopes of collagen II with minimal cross-reactivity to other collagen types. The native-conformation reagent for cartilage collagen-II ELISA (use cat. 210 human collagen II antigen as the standard) and frozen-section IHC — cartilage biology, osteoarthritis and chondrogenesis research. Not suitable for denaturing conditions.
SPECIFICATIONS
Catalogue number: 214 | Host: Rabbit | Target: Human
Target antigen: Native human collagen type II (COL2A1), triple-helical fold; cartilage collagen
Gene: COL2A1 (12q13.11) | UniProt: P02458
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds native 3-D epitopes of human collagen II; minimal cross-reactivity to other human collagen types (cross-absorbed). Native-conformation — not suitable for denaturing / dissociating conditions.
Purification: Affinity-purified on immobilised human collagen II, cross-absorbed against other human collagens.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol | Package size: 0.5 mL (reconstitute in 0.5 mL sterile water)
STORAGE / RECONSTITUTION
Reconstitute with 0.5 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection of human collagen II (starting dilution 1:3 000); use cat. 210 (human collagen II antigen) as the immobilised standard.
- IHC (frozen sections) — cartilage collagen-II histochemistry (starting dilution 1:20); no antigen retrieval typically required.
- Cell culture / ECM staining — collagen-II matrix deposited by chondrocytes and chondrogenic cultures.
- Note: native-conformation antibody — not suitable for denaturing / dissociating conditions (no reducing Western blot).
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Cartilage biology and chondrogenesis — collagen-II as the definitive hyaline-cartilage marker.
- Osteoarthritis / cartilage-degradation research.
- Cartilage tissue engineering and MSC chondrogenic-differentiation QC.
- Intervertebral-disc and growth-plate research.
