Polyclonal goat antibody
anti-human collagen type III (COL3A1)
Specificity
Binds native 3D epitopes
of human collagen type III. Cross-reactivity to human collagen type
I, II, IV and V <5%. No cross-reactivity to human plasma proteins
Preparation
Affinity purification on
immobilized human collagen type III with cross-absorption using
immobilised human plasma proteins and human collagen type I, II, IV
and IV. Overall process is designed to obtain a high purity
type-specific antibody to native 3D collagen.
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1%mannitol, pH 8
Applications and
recommended dilutions
Detection of human
collagen type III by ELISA (x3000), Histochemistry (frozen sections)
(x20). Immunostaining of ECM in cell culture. Not suitable for
denaturing and dissociating conditions
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibody 2 years at -20°C or
lower
