Mouse monoclonal antibody against human collagen type III (COL3A1), clone MC3-HA, 1 mL. The natural sandwich-ELISA capture partner for cat. 153 (goat anti-collagen III polyclonal) and cat. 661 (rabbit anti-collagen III polyclonal). The mAb for routine FFPE collagen-III IHC (where the native-conformation polyclonals underperform), denatured-Western-blot detection of collagen-III a-chains, and immunoprecipitation of collagen III from cell-conditioned media. Pairs with cat. 889 (human collagen III antigen, placenta-derived) as the antigen-capture combination.
SPECIFICATIONS
Catalogue number: 380 | Host: Mouse | Target: Human
Target antigen: Human collagen type III (COL3A1), a1 chain
Gene: COL3A1 | UniProt: P02461
Clonality: Monoclonal | Clone: MC3-HA | Conjugate: Unconjugated
Specificity: Human collagen III a1 chain; FFPE-compatible after HIER and denatured-Western-blot-compatible.
Format: Standard YO formulation | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute and handle per the supplied protocol. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — sandwich-ELISA capture arm; pair with cat. 153 (goat polyclonal) or cat. 661 (rabbit polyclonal) on detection, and use cat. 889 (human collagen III antigen) as the standard.
- IHC (FFPE) — collagen-III IHC on paraffin-embedded tissue (HIER, citrate pH 6.0); the FFPE-compatible alternative to native-conformation polyclonals.
- IHC (frozen sections) — also compatible.
- Western blot — denatured / reducing conditions; detects the collagen-III a1 chain.
- Immunoprecipitation — couple to Protein G beads.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Sandwich-ELISA capture — as used in the Capossela intervertebral-disc autoantibody panel.
- FFPE collagen-III IHC — archival fibrosis pathology (dermal, hepatic, pulmonary, renal, cardiac).
- Denatured Western-blot collagen-III detection in cell-culture lysate workflows.
- Tumour-stroma collagen-III IHC by cancer-associated fibroblasts.
- Vascular Ehlers-Danlos research (COL3A1 mutations).
REFERENCES
Capossela S, Schläfli P, Bertolo A, Janner T, Stadler BM, Pötzel T, Baur M, Stoyanov JV. (2014) Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins. Eur Cell Mater. 27:251-63.
