Specificity
Binds human Collagen type
III. No cross-reactivity to other collagen types and human plasma
proteins
Preparation
PEG-6000 precipitation and
purification by gel chromatography
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1%mannitol, pH 8
Applications and
recommended dilutions
ELISA (x1000),
Immunohistology (x20). Not suitable for denaturing conditions
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibody 2 years at -20°C or
lower. Storage of reconstituted antibodies 10 days at +4°C.
References
Capossela S, Schläfli P, Bertolo A, Janner T, Stadler BM, Pötzel T, Baur M, Stoyanov JV. (2014) Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins. Eur Cell Mater. 27:251-63
