Rabbit polyclonal antibody against human collagen type III, affinity-purified and cross-adsorbed, 1 mL. Binds native human collagen type III with no cross-reactivity to human collagen I, II, IV or V, or to human plasma proteins. A type-III-specific reagent for native-conformation immunoassay and histochemistry: pair with the monoclonal cat. 380 (mouse anti-human collagen III, clone MC3-HA) in sandwich ELISA and use cat. 889 (human collagen III antigen) as the standard; the rabbit-host alternative to the goat polyclonal cat. 153.
SPECIFICATIONS
Catalogue number: 661 | Host: Rabbit | Target: Human
Target antigen: Native human collagen type III
Gene: COL3A1 (2q32.2) | UniProt: P02461
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds native human collagen type III; no cross-reactivity to human collagen I, II, IV or V, or to human plasma proteins. Native-conformation — not suitable for denaturing or dissociating conditions.
Purification: Affinity chromatography on immobilised human collagen type III, cross-adsorbed against immobilised human plasma proteins and human collagen I, II, IV and V.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — native human collagen III immunoassay (dilution x3000). Pair with the monoclonal cat. 380; use cat. 889 (human collagen III antigen) as the standard.
- IHC (frozen sections) — human collagen III histochemistry (dilution x20).
- Note: native-conformation antibody — not suitable for denaturing or dissociating conditions.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Fibrosis research — collagen III deposition in hepatic, pulmonary, renal and cardiac fibrosis.
- Wound healing and scarring — early collagen III to collagen I turnover in repair.
- Vascular biology — collagen III in vessel-wall integrity and aneurysm research.
- Connective-tissue disease — collagen III in Ehlers-Danlos-type and matrix disorders.
- Tumour stroma — collagen III in the desmoplastic reaction.
