Polyclonal rabbit antibody
anti-human Collagen type IV (placenta)
Specificity
Binds human Collagen type IV. Cross-reactivity to other human collagen
type I, II, III and V below 5%. No cross-reactivity to human plasma
proteins
Preparation
Affinity purification on
immobilized collagen type IV with cross-absorption to immobilised
human blood plasma proteins and human collagen type I, II, III and V.
Overall process is designed to obtain a high purity type-specific
antibody to native 3D collagen
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1%mannitol, pH 8
Applications and
recommended dilutions
ELISA (x3000),
Histochemistry (frozen sections) (x20). Not suitable for denaturing
and dissociating conditions
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies for 2 years at -20°C
or lower
Citations
Hahn JM, Combs KA, Lloyd CM, McFarland KL, Boyce ST, Supp DM (2019) Identification of Merkel cells associated with neurons in engineered skin substitutes after grafting to full thickness wounds. PLoS ONE 14(3): e0213325.
Hahn JM, McFarland KL, Combs KA, Powell HM, Supp DM. Myofibroblasts Are Not Characteristic Features of Keloid Lesions. Plast Reconstr Surg Glob Open. 2022 Nov 28;10(11):e4680. doi: 10.1097/GOX.0000000000004680. PMID: 36448015; PMCID: PMC9699581.
