Rabbit polyclonal antibody against human collagen type IV (placenta), affinity-purified and cross-adsorbed, 1 mL. Binds native human collagen type IV with less than 5% cross-reactivity to human collagen I, II, III or V and no reactivity to human plasma proteins. A type-IV-specific reagent for native-conformation immunoassay and histochemistry — pair as the polyclonal detection arm with the monoclonal cat. 533 (mouse anti-human collagen IV, clone MC4-HA) and use cat. 425 (human collagen IV antigen) as the ELISA standard.
SPECIFICATIONS
Catalogue number: 507 | Host: Rabbit | Target: Human
Target antigen: Human collagen type IV (placenta)
Gene family: COL4 (COL4A1 / COL4A2)
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds human collagen type IV; cross-reactivity to human collagen I, II, III and V below 5%; no cross-reactivity to human plasma proteins. Native-conformation — not suitable for denaturing or dissociating conditions.
Purification: Affinity chromatography on immobilised collagen type IV, cross-adsorbed against immobilised human plasma proteins and human collagen I, II, III and V.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — native human collagen IV immunoassay (dilution x3000). Pair with the monoclonal cat. 533; use cat. 425 (human collagen IV antigen) as the standard.
- IHC (frozen sections) — human collagen IV histochemistry (dilution x20).
- Note: native-conformation antibody — not suitable for denaturing or dissociating conditions.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Basement-membrane biology — collagen IV mapping in vascular and epithelial basement membranes.
- Renal research — glomerular basement-membrane collagen IV in nephropathy and Alport-type studies.
- Tumour invasion — basement-membrane collagen IV breakdown at the invasive front.
- Angiogenesis — vascular basement-membrane remodelling.
- Fibrosis — collagen IV deposition in tissue remodelling.
