Monoclonal mouse antibody
(clone MC5-HA, IgM fraction) anti-human Collagen type V
Specificity
Binds Human Collagen type
V in native conformation as well as heat denatured human collagen
type V in RIA, ELISA and immunoblot assays. The antibody does not
react with other collagen types I, II, III, and IV. Human plasma
proteins do not interfere with binding to collagen.
Preparation
PEG-6000 precipitation and
purification by gel chromatography
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1%mannitol, pH 8
Applications and
recommended dilutions
ELISA (x1000),
Immunohistology (x20)
Reconstitution
Use 1 ml sterile pure
water
Storage
Storage of lyophilized
antibodies 2 years -20°C or lower. Storage of reconstituted
antibodies 10 days at +4°C