Mouse IgM monoclonal antibody against human fibrin / fibrinogen, clone MFB-HB, 1 mL. Recognises both human and canine fibrin and fibrinogen, with no affinity to other human plasma proteins. Native-conformation reagent for fibrin(ogen) detection in ELISA, frozen-section IHC and enzyme immunoassay — thrombosis, clot / thrombus imaging, wound-healing and tumour-associated fibrin research.
SPECIFICATIONS
Catalogue number: 278 | Host: Mouse | Target: Human (and canine)
Target antigen: Human fibrin and fibrinogen (also reacts with canine fibrin / fibrinogen)
Gene: FGA / FGB / FGG | UniProt: P02671, P02675, P02679
Clonality: Monoclonal | Clone: MFB-HB | Isotype: Mouse IgM | Conjugate: Unconjugated
Specificity: Human and canine fibrin and fibrinogen; no affinity to other human plasma proteins.
Purification: PEG 6000 precipitation and DEAE-cellulose chromatography.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol | Package size: 1 mL (reconstitute in 1 mL sterile water)
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — fibrin(ogen) detection (1:1 000).
- IHC (frozen sections) — fibrin / fibrinogen deposition in tissue.
- Enzyme immunoassay (EIA) — fibrin(ogen) quantification.
- Note: IgM isotype — use an anti-mouse-IgM secondary for detection.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Thrombosis / haemostasis research — fibrin clot formation and fibrinogen deposition.
- Thrombus / clot imaging and detection.
- Wound-healing and provisional-matrix fibrin research.
- Tumour-associated fibrin and canine (veterinary) thrombosis models.
