Rabbit polyclonal antibody against human fibronectin, affinity-purified on immobilised human fibronectin and cross-adsorbed against human plasma proteins, 0.1 mL. Binds human fibronectin with no affinity to other human plasma proteins. The native-conformation reagent for fibronectin ELISA and frozen-section IHC — ECM assembly, wound healing, fibrosis and cell-adhesion research.
SPECIFICATIONS
Catalogue number: 770 | Host: Rabbit | Target: Human
Target antigen: Human fibronectin (plasma and cellular forms)
Gene: FN1 (2q35) | UniProt: P02751
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds human fibronectin; no affinity to other human plasma proteins (cross-adsorbed).
Purification: Affinity-purified on immobilised human fibronectin, with cross-adsorption on immobilised human plasma proteins.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol | Package size: 0.1 mL (reconstitute in 0.1 mL sterile water)
STORAGE / RECONSTITUTION
Reconstitute with 0.1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection of human fibronectin (starting dilution 1:3 000).
- IHC (frozen sections) — fibronectin localisation in tissue (starting dilution 1:20).
- Cell culture / ECM staining — fibronectin fibrillogenesis and matrix assembly by fibroblasts.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- ECM assembly and fibrillogenesis — fibronectin as the master matrix organiser.
- Wound healing — provisional fibronectin matrix in granulation tissue.
- Fibrosis research — fibronectin (incl. EDA-FN) deposition preceding collagen.
- Cell adhesion, migration and integrin biology.
