Sheep polyclonal antibody against human IgA, affinity-purified and cross-adsorbed, 1 mg. Highly IgA-specific — 100% reactivity to human IgA with less than 10% to human IgG, less than 5% to human IgM and less than 1% to human serum albumin. A class-specific detection reagent for human IgA, complementing the anti-IgG reagent cat. 493 and the pan-immunoglobulin cat. 172 (rabbit anti-human IgG + IgM + IgA).
SPECIFICATIONS
Catalogue number: 541 | Host: Sheep | Target: Human
Target antigen: Human immunoglobulin A (IgA)
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Human IgA 100%; human IgG below 10%, human IgM below 5%, human serum albumin below 1%.
Purification: Affinity chromatography on immobilised IgA, cross-adsorbed over human plasma proteins.
Format: Aqueous solution — 1 mg/mL antibody in 50% glycerol, 0.05 M sodium phosphate, pH 7.5 | Package size: 1 mg
STORAGE
Liquid glycerol solution — no reconstitution required. Ships at ambient temperature. Store at -20 °C or lower. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection of human IgA (dilution x10000).
- Western blotting — detection of human IgA.
- Immunohistochemistry — detection of human IgA in tissue.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Mucosal immunology — secretory and serum IgA in gut, respiratory and glandular tissue.
- IgA nephropathy — IgA deposition in renal biopsy studies.
- Serology — IgA-class antibody responses to infection and vaccination.
- Coeliac and autoimmune research — IgA autoantibody detection.
- Immunoassay development — class-specific human IgA detection reagent.
