Mouse IgG1 monoclonal antibody against the human IgA a-chain, clone BFG 267, 1 mL. IgA-specific — no reactivity to human IgE, IgG, IgM or other serum proteins. The monoclonal capture / detection partner for isotype-specific human-IgA immunoassays; pair with the polyclonal anti-human IgA reagents cat. 315 (goat) and cat. 395 (rabbit) for two-site sandwich ELISA.
SPECIFICATIONS
Catalogue number: 807 | Host: Mouse | Target: Human
Target antigen: Human IgA (a-chain)
Clonality: Monoclonal | Clone: BFG 267 | Isotype: Mouse IgG1 | Conjugate: Unconjugated
Specificity: Specific for the human IgA a-chain; no reactivity to human IgE, IgG, IgM or other serum proteins.
Purification: Sodium sulphate precipitation and anion-exchange chromatography (DEAE-cellulose).
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol | Package size: 1 mL (reconstitute in 1 mL sterile water)
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water; aliquot and store at -20 °C or lower. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — isotype-specific human-IgA capture or detection (dilution 1:1 000); pair with the polyclonal cat. 315 or cat. 395 on the opposite arm for a two-site sandwich assay.
- Immunohistochemistry — IgA-plasma-cell and mucosal-IgA detection (dilution 1:40).
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Mucosal immunology — secretory-IgA quantification.
- IgA nephropathy research — IgA-deposition detection.
- Isotype-specific serology — IgA-class antibody titres.
- Two-site IgA sandwich immunoassay development (mAb capture + polyclonal detection).
