Goat polyclonal antibody against human IgG, affinity-purified and cross-adsorbed, 1 mg. Reacts with human IgG with no reactivity to human IgA, IgM or other serum proteins — an isotype-clean secondary/detection reagent for human IgG. For broader anti-immunoglobulin reactivity see the rabbit cat. 172 (anti-human IgG + IgM + IgA), or for the IgM-specific counterpart the goat cat. 321 (anti-human IgM).
SPECIFICATIONS
Catalogue number: 454 | Host: Goat | Target: Human
Target antigen: Human IgG
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Reacts with human IgG; no reactivity to human IgA, IgM or other serum proteins.
Purification: Affinity chromatography on immobilised IgG; cross-adsorbed against human IgA, IgM and other serum proteins
Format: Solution, 1 mg/mL antibody in 50% glycerol, 0.1 M NaCl, 0.05 M sodium phosphate, pH 7.5 | Package size: 1 mg
STORAGE
Ships at ambient temperature. Store 2 years at -20 °C or lower. The 50% glycerol formulation allows storage at -20 °C without freezing; avoid repeated freeze-thaw.
APPLICATIONS
- ELISA (dilution x1000) — detection of human IgG.
- Western blot — secondary detection of human IgG.
- Immunohistochemistry — localisation of human IgG in tissue.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Human serology — isotype-specific IgG detection in infection and vaccine-response assays.
- Autoimmunity — quantification of human IgG autoantibodies.
- Immunoassay development — cross-adsorbed detection arm with no IgA/IgM cross-reactivity.
- Immune-complex & deposition studies — human IgG detection in tissue by IHC.
- Therapeutic-antibody / IgG monitoring — detection of human IgG in research and QC settings.
