Rabbit polyclonal antibody against human immunoglobulin G (IgG), affinity-purified and cross-adsorbed, 1 mg. Specific for human IgG with no reactivity to human IgA, IgM or other serum proteins. A detection and immunoassay reagent for human IgG — the class-specific complement to the pan-immunoglobulin cat. 172 (rabbit anti-human IgG + IgM + IgA) and the polyclonal alternative to the monoclonal cat. 534 (mouse anti-human IgG, clone EFE-565).
SPECIFICATIONS
Catalogue number: 493 | Host: Rabbit | Target: Human
Target antigen: Human immunoglobulin G (IgG)
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds human IgG; no reactivity to human IgA, IgM or other serum proteins.
Purification: Affinity chromatography on immobilised IgG; cross-adsorbed against human IgA, IgM and other serum proteins.
Format: Solution — 1 mg/mL antibody in 50% glycerol, 0.1 M sodium chloride, 0.01 M sodium phosphate, pH 7.5 | Package size: 1 mg
STORAGE
Liquid glycerol solution — no reconstitution required. Ships at ambient temperature. Store 2 years at -20 °C or lower. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection of human IgG (dilution x1000).
- Western blotting — detection of human IgG.
- Immunohistochemistry — detection of human IgG in tissue.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Clinical immunology — quantification of human IgG in serum and biological fluids.
- Serology — detection of IgG-class antibody responses in autoimmune and infectious disease.
- Immune-complex studies — IgG deposition in tissue and circulating complexes.
- Bioprocess / antibody engineering QC — detection of human IgG in expression and purification workflows.
- Multiplex immunoassays — cross-adsorbed reagent for class-specific human IgG detection.
