Mouse monoclonal antibody against human IgG (gamma chain), clone EFE-565 (IgG1), 1 mL. Specific for the gamma heavy chain of human IgG with no cross-reaction to human IgA, IgE, IgM or other plasma proteins. A monoclonal detection reagent for human IgG — the defined-clone alternative to the polyclonal cat. 493 and the class-specific complement to the pan-immunoglobulin cat. 172 (rabbit anti-human IgG + IgM + IgA).
SPECIFICATIONS
Catalogue number: 534 | Host: Mouse | Target: Human
Target antigen: Human immunoglobulin G (IgG), gamma chain
Clonality: Monoclonal | Clone: EFE-565 | Isotype: IgG1 | Conjugate: Unconjugated
Specificity: Binds the gamma heavy chain of human IgG; no cross-reaction to human IgA, IgE, IgM or other plasma proteins.
Purification: Sodium sulphate precipitation followed by DEAE-cellulose ion-exchange chromatography.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection of human IgG.
- Western blotting — detection of human IgG gamma chain.
- Immunohistochemistry — detection of human IgG in tissue.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Clinical immunology — defined-clone detection of human IgG in serum and biological fluids.
- Serology — IgG-class antibody responses in autoimmune and infectious disease.
- Therapeutic antibody / bioprocess QC — monitoring human IgG in expression and purification workflows.
- Immunoassay development — reproducible monoclonal capture or detection arm for human IgG.
- Gamma-chain-specific detection — discrimination of IgG from other immunoglobulin classes.
