Polyclonal goat antibody
anti-human immunoglobulin IgG conjugated to horseradish peroxidase HRP (affinity
purified and cross-adsorbed)
Specificity
Human immunoglobulin IgG.
No reactivity against human IgA, IgM, other serum proteins
Preparation
Purification by affinity
chromatography using immobilized IgG. Cross-adsorption against human
IgA, IgM and other serum proteins. Purification by gel filtration and
anion-exchange chromatography to remove protein aggregates and free
peroxidase
Format
Antibody solution 1.2
mg/ml in the buffer (0.1 M sodium chloride, 0.01M sodium phosphate pH
7.5, 50% glycerol, 5 mg/ml BSA)
Applications and
recommended dilutions
ELISA (x10000),
Immunohistology, Blotting. Dilution should be adjusted to the
appropriate task. Do not use azide together with HRP-conjugated products since azide strongly inhibits horseradish peroxidase (HRP)
Storage
Shipping at ambient
temperature. Storage at –20°C for 1 year