Goat polyclonal anti-Mouse IgG + IgM + IgA, cross-adsorbed against human Ig and murine plasma albumin and affinity-purified on the immobilised mixed-isotype antigen. Liquid format (1 mg/mL in PBS + 50 % glycerol). 100 % reactivity for mouse IgG, 90 % for IgM, 85 % for IgA; < 5 % cross-reactivity to human IgG / IgM / IgA. The unconjugated H+L pan-isotype reagent for mouse total-Ig sandwich-ELISA capture, hybridoma-supernatant screening, mouse-model autoimmunity panels, and custom HRP / biotin / Alexa Fluor conjugation.
Specifications
Catalogue number: 133
Product name: Goat anti-Mouse IgG, IgM, IgA (H+L) polyclonal antibody — cross-adsorbed, affinity-purified
Host species: Goat
Target species: Mouse (Mus musculus)
Reactivity / class: Mouse IgG (heavy + light chain), IgM, IgA
Clonality: Polyclonal
Conjugate: Unconjugated (suitable for custom conjugation and as ELISA capture)
Specificity (ELISA, normalised to mouse IgG = 100 %):
• Mouse IgG: 100 %
• Mouse IgM: 90 %
• Mouse IgA: 85 %
• Murine plasma albumin: < 0.5 %
• Human IgG / IgM / IgA: < 5 %
Cross-adsorption: Cross-adsorbed against human IgG / IgM / IgA and murine plasma albumin
Preparation: Affinity-purified on immobilised mouse IgG / IgM / IgA mixed-isotype antigen
Format: Liquid, 1 mg/mL in 50 mM sodium phosphate pH 7.4, 0.1 M NaCl, 50 % glycerol
Package size: 1 mg (1 mL at 1 mg/mL)
Applications
ELISA capture antibody (mouse total-Ig)
• Coat at 1–5 µg/mL in carbonate / bicarbonate coating buffer pH 9.6.
• For detection-arm ELISA, dilute 1:30 000 in blocking / sample buffer.
• Validated in hybridoma-supernatant screening and mouse-serum titration.
ELISA detection (in combination with HRP-conjugated secondary)
• 1:30 000 dilution as above; use a downstream anti-goat HRP for detection
(YO cat. 319 — rabbit anti-goat IgG, IgM, IgA HRP, 1.2 mg).
Western blot
• 1:1 000 to 1:5 000 dilution in TBS-T + 5 % non-fat milk or 5 % BSA.
• Use as the detection arm against blotted mouse mAb, blotted mouse plasma/serum samples, or hybridoma-secreted mouse Ig.
Immunohistochemistry / Immunofluorescence
• Titrate locally; typical starting dilution 1:200 to 1:1 000.
• Pair with an anti-goat fluorophore secondary or detection system.
Custom conjugation starting material
• The 50 % glycerol, sodium-phosphate / NaCl liquid format is compatible with standard NHS-ester (HRP, biotin), maleimide (cysteine-thiol), and amine-directed Alexa Fluor / DyLight conjugation chemistries after a buffer-exchange step into the kit-specified reaction buffer.
Immunoprecipitation
• The unconjugated affinity-purified format is preferred over HRP / biotin conjugates for IP applications — couple to Protein A / G beads or use as a matrix-bound mouse-Ig capture reagent.
Hybridoma-supernatant screening
• Coat 96-well plates at 1 µg/mL; capture secreted mouse Ig in supernatant; detect with isotype-specific HRP secondary for class profiling.
Mouse autoimmunity / total-Ig panels
• Use as capture for total-Ig quantification in mouse serum; pair with isotype-specific detection (anti-IgG only / anti-IgM only / anti-IgA only) to compute class distributions.
Storage
Shipped at ambient temperature.
Liquid antibody (1 mg/mL in PBS + 50 % glycerol): store up to 2 years at -20 °C or lower.
