Sheep polyclonal antibody against rabbit immunoglobulins IgG, IgM and IgA, affinity-purified, 1 mg. Recognises rabbit IgG, IgM and IgA with no cross-reactivity to other rabbit plasma proteins. A secondary detection reagent for rabbit primary antibodies — pairs with the rabbit polyclonals in this catalogue such as cat. 507 (rabbit anti-human collagen IV) and cat. 553 (rabbit anti-human ApoA1); one of a panel of species-specific anti-immunoglobulin polyclonals alongside cat. 531 (anti-bovine) and cat. 172 (anti-human).
SPECIFICATIONS
Catalogue number: 596 | Host: Sheep | Target: Rabbit
Target antigen: Rabbit immunoglobulins IgG, IgM and IgA
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds rabbit IgG, IgM and IgA; no cross-reactivity to other rabbit plasma proteins.
Purification: Affinity chromatography on rabbit IgG, IgM and IgA coupled to sorbent.
Format: Liquid formulation — 1 mg/mL antibody in 50% glycerol, 0.1 M sodium chloride, 10 mM sodium phosphate, pH 7.5 | Package size: 1 mg
STORAGE
Liquid glycerol formulation — no reconstitution required. Ships at ambient temperature. Store 2 years at -20 °C or lower. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — secondary detection of rabbit immunoglobulins (dilution 1:1000).
- Western blotting — secondary detection of rabbit primary antibodies.
- Dot blotting — detection of rabbit immunoglobulins (dilution 1:100).
- Immunohistochemistry — secondary detection of rabbit primaries in tissue sections.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Universal secondary reagent — detection arm for any rabbit primary antibody workflow.
- Sandwich immunoassay build-out — sheep-host secondary avoids conflict with mouse and goat primaries.
- Rabbit immunology — quantification of rabbit antibody responses to immunisation.
- Multiplex assay design — species-specific detection in mixed-primary panels.
- Hybridoma and antiserum QC — titre determination of rabbit polyclonal preparations.
