Rabbit polyclonal antibody against rat IgG + IgM + IgA, affinity-purified on immobilised rat Ig, 1 mg. Pan-class rat-Ig secondary reagent — reacts with rat IgG, IgM and IgA with no cross-reactivity to other rat plasma proteins. Unconjugated liquid (glycerol) format for rat total-Ig ELISA, Western blot, dot blot, immunohistochemistry and custom conjugation.
SPECIFICATIONS
Catalogue number: 816 | Host: Rabbit | Target: Rat (Rattus norvegicus)
Target antigen: Rat IgG, IgM and IgA — mixed-isotype immunogen
Reactivity: Rat IgG, IgM and IgA
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Pan-class rat Ig; no cross-reactivity to other rat plasma proteins.
Purification: Affinity chromatography on immobilised rat IgG / IgM / IgA.
Format: Liquid, 1 mg/mL in 50 mM sodium phosphate, 0.1 M NaCl, 50 % glycerol | Package size: 1 mg
STORAGE
Ships at ambient temperature. Store at -20 °C or lower; the 50 % glycerol keeps the antibody liquid at -20 °C, so there are no freeze-thaw concerns. Avoid microbial contamination.
APPLICATIONS
- ELISA — rat total-Ig capture or detection (1:1 000).
- Western blot — detection of rat Ig and rat primary antibodies.
- Dot blot — 1:100.
- Immunohistochemistry — rat Ig detection in tissue; secondary for rat primary antibodies.
- Custom conjugation — compatible with HRP / biotin / fluorophore labelling.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Secondary reagent for rat primary antibodies — detection chain in ELISA, WB and IHC.
- Rat-model immunology and autoimmunity studies — total-Ig quantification in rat sera.
- Rat hybridoma-supernatant screening.
- Custom-conjugate starting material for anti-rat detection reagents.
