Polyclonal sheep antibody
anti-rabbit immunoglobulins IgG, IgM, IgA conjugated to FITC
Specificity
Binds rabbit
immunoglobulins IgG, IgM, IgA. Affinity to human IgM, IgG, IgA as
measured by ELISA below 3%
Preparation
Purification by affinity
chromatography using antigen coupled to sorbent. Antibody
concentration was determined by extinction coefficient prior to
conjugation to fluorescein isothiocyanate (FITC). Chromatographic
separation of free fluorescein and protein aggregates. Labeling four
molecules FITC per antibody
Format
Solution 1.2 mg/mL
antibody, 0.05 M sodium phosphate pH 7.5, 0.1 M NaCl, 50% glycerol, 5
mg/ml BSA
Applications and
recommended dilutions
Western blotting, ELISA,
Immunohistology (1:200), Cell staining, Cell sorting, Electron
microscopy
Storage
Shipping at ambient
temperature. Storage at –20°C for 2 years
Citations
1. Ehlicke, F., Freimark, D., Heil, B., Dorresteijn, A., Czermak, P. (2010). Intervertebral disc regeneration: Influence of growth factors on differentiation of human mesenchymal stem cells (hMSC). International Journal of Artificial Organs, 33 (4), 244-252.
2. Lee E, Choi J, Lee HS (2017) Palmitate induces mitochondrial superoxide generation and activates AMPK in podocytes. J. Cellular Physiology 232:12:3209-3217
