Sheep immunoglobulins IgG,
IgM, IgA. No cross-reactivity to other sheep plasma proteins.
Purification by affinity
chromatography using antigen coupled to sorbent. Antibody
concentration was determined by extinction coefficient prior to
conjugation to fluorescein isothiocyanate (FITC). Chromatographic
separation of free fluorescein and protein aggregates.
Labeling four molecules FITC per antibody
Antibody solution 1.2
mg/ml in the buffer (0.05 M sodium phosphate pH 7.5, 0.1 M NaCl, 50%
glycerol, 5 mg/ml BSA)
Western blotting, ELISA,
Immunohistology (1:40), Cell staining, Cell sorting, Electron microscopy
Shipping at ambient
temperature. Storage at –20°C for 2 years