Polyclonal goat antibody anti-rabbit immunoglobulins IgG, IgM, IgA conjugated to horseradish peroxidase (HRP)
Specificity
Rabbit immunoglobulins IgG, IgM, IgA
Preparation
Purification by affinity chromatography using antigen coupled to sorbent. Purification by gel filtration and anion-exchange chromatography to remove protein aggregates and free peroxidase.
Format
Antibody solution 1.2 mg/ml in the buffer (0.05M sodium phosphate pH 7.5, 0.1 M NaCl, 50% glycerol, 5 mg/ml BSA)
Applications and recommended dilutions
Suitable for chemiluminescent detection of rabbit polyclonal and monoclonal antibodies of IgG, IgM and IgA.
ELISA (1), Immunohistology, Blotting. Dilution should be adjusted to the appropriate task. Do not use azide together with HRP-conjugated products since azide strongly inhibits horseradish peroxidase (HRP)
Storage
Shipping at ambient temperature. Storage for 1 year at -20°C or lower
Citations
1. Irazoki O, Campoy S, Barbe J (2017) The Transient Multidrug Resistance Phenotype of Salmonella enterica Swarming Cells Is Abolished by Sub-inhibitory Concentrations of Antimicrobial Compounds. Front Microbiol. 2017; 8: 1360
2. Jordi Corral, María Pérez-Varela, Jordi Barbé & Jesús Aranda (2020) Direct interaction between RecA and a CheW-like protein is required for surface-associated motility, chemotaxis and the full virulence of Acinetobacterbaumannii strain ATCC 17978, Virulence, 11:1,
315-326, DOI: 10.1080/21505594.2020.1748923
