Polyclonal rabbit antibody
anti-mouse (murine) immunoglobulins IgG, IgM, IgA conjugated to
horseradish peroxidase (HRP)
Specificity
Murine immunoglobulins
IgG, IgM, IgA. No cross-reactivity to other mouse plasma proteins.
Preparation
Purification by affinity
chromatography using immobilized mouse IgG, IgM, IgA. Purification
by gel filtration and anion-exchange chromatography to remove protein
aggregates and free peroxidase.
Format
Antibody solution 1.2
mg/ml in the buffer (0.05M sodium phosphate pH 7.5, 0.1 M NaCl, 50%
glycerol, 5 mg/ml BSA)
Applications and
recommended dilutions
ELISA (x1000),
Immunohistochemistry, Blotting. Dilution should be adjusted to the
appropriate task. Do not use azide together with HRP-conjugated products since azide strongly inhibits horseradish peroxidase (HRP)
Storage
Shipping at ambient
temperature. Storage for 1 year at -20°C or lower
Citations
1. Wahlgren J, De L Karlson T, Brisslert M, Vaziri Sani F, Telemo E, Sunnerhagen P, Valadi H. Plasma exosomes can deliver exogenous short interfering RNA to monocytes and lymphocytes. Nucleic Acids Res. 2012 Sep 1;40(17):e130. Epub 2012 May 22. PMID: 22618874
2. Kleinschmidt M, Schoenfeld R, Göttlich C, Bittner D, Metzner JE, Leplow B, Demuth H-U (2016) Characterizing Aging, Mild Cognitive Impairment, and Dementia with Blood-Based Biomarkers and Neuropsychology. Journal of Alzheimer's Disease 50:111-126
