Polyclonal rabbit antibody
anti-human IgM conjugated to horseradish peroxidase (HRP)
(cross-adsorbed and affinity purified)
Specificity
Human immunoglobulin IgM.
No reactivity against human IgG, IgA, other serum proteins
Preparation
Purification by affinity
chromatography using immobilized IgM. Cross-adsorption against human
IgG, IgA and other serum proteins. Purification by gel filtration and
anion-exchange chromatography to remove protein aggregates and free
peroxidase.
Format
Solution 1.2 mg/mL
antibody, 50% glycerol, 0.1M NaCl, 0.05M sodium phosphate pH 7.5, 5
mg/ml BSA
Applications and
recommended dilutions
ELISA (x10000),
Immunohistochemistry, Blotting. Dilution should be adjusted to the
appropriate task. Do not use azide together with HRP-conjugated products since azide strongly inhibits horseradish peroxidase (HRP)
Storage
Shipping at ambient
temperature. Storage for 1 year at -20°C or lower
Citations
Kleinschmidt M, Schoenfeld R, Göttlich C, Bittner D, Metzner JE, Leplow B, Demuth H-U (2016) Characterizing Aging, Mild Cognitive Impairment, and Dementia with Blood-Based Biomarkers and Neuropsychology. Journal of Alzheimer's Disease 50:111-126