Polyclonal rabbit antibody
anti-human urokinase (abbokinase, urokinase-type Plasminogen
Activator, uPA, PLAU)
Specificity
The antibody binds to
human HMW-scuPA (54 kDa), HMW-tsuPA (52 kDa) and LMW-scuPA (33 kDa)
with high apparent affinity (Kd= 0.1 nM) completely inhibiting the
ability of urokinase to activate plasminogen. Urokinase reaction with
synthetic substrate S2444 is also inhibited. The antibody is highly
specific for urokinase, no crossreactions were found with other
serine proteases (tissue plasminogen activator, plasmin, trypsin,
chymotrypsin, elastase, thrombin). Human whole plasma or plasma
proteins do not interfere with its binding to urokinase.
Preparation
Affinity purification with
immobilized human urokinase. Cross-adsorption on immobilised human
plasma proteins
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1%mannitol, pH 8
Applications and
recommended dilutions
ELISA (x5000), IHC (x50)
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower
