Mouse IgG1 monoclonal antibody against human urokinase-type plasminogen activator (uPA / PLAU; abbokinase), clone PGM2005, 1 mL. Pan-form — recognises HMW single-chain (54 kDa), HMW two-chain (52 kDa) and LMW (33 kDa) uPA. The epitope-distinct partner to cat. 381 (clone PGM2001) for two-mAb sandwich ELISA on the same antigen, and complements the neutralising polyclonal cat. 168. For uPA IHC, EIA / ELISA, functional studies, Western blot, RIA and affinity purification.
SPECIFICATIONS
Catalogue number: 335 | Host: Mouse | Target: Human
Target antigen: Urokinase-type plasminogen activator (uPA / PLAU; abbokinase)
Gene: PLAU (10q24.33) | UniProt: P00749
Clonality: Monoclonal | Clone: PGM2005 | Isotype: Mouse IgG1 | Conjugate: Unconjugated
Specificity: Binds HMW-scuPA (54 kDa), HMW-tcuPA (52 kDa) and LMW-scuPA (33 kDa) — all uPA forms.
Purification: PEG-6000 precipitation and DEAE-cellulose chromatography.
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M NaCl, 1% dextran, 1% mannitol | Package size: 1 mL (reconstitute in 1 mL sterile water)
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower; store reconstituted antibody at +4 °C up to 4 weeks, or aliquot and freeze at -20 °C. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA / EIA / RIA — uPA detection; pair with cat. 381 (clone PGM2001, epitope-distinct) for two-mAb sandwich assays, or with the polyclonal cat. 168.
- IHC (frozen sections) — uPA in tumour invasive fronts and other tissue.
- Western blot — detects HMW-scuPA (54 kDa), HMW-tcuPA (52 kDa) and LMW-uPA (33 kDa).
- Functional studies — uPA activity assays.
- Affinity purification — column ligand for uPA capture.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Cancer invasion / metastasis research — pan-form uPA detection.
- Two-mAb sandwich ELISA (PGM2005 / PGM2001) for uPA quantification.
- Fibrinolysis and thrombosis research.
- Tumour uPA / PAI-1 biomarker IHC.
