Full length native
Apolipoprotein A1 protein purified from human plasma
Source
Human plasma. Donors
tested negative for HBsAg, HBc, HIV-I, HIV-II and HCV antibodies
Purity
At least 90% pure, judged
by SDS-PAGE
Format
Lyophilized from 0.05 M
Na-chloride, 0.01 M Na-carbonate, pH 10.0
Purification
Ultracentrifugation in
density gradient. Delipidation with diethyl ether and ethanol. Gel
filtration on Sephacryl S-300
Applications
Apolipoprotein A1
standard. Antigen for immunisation (1) and antibody production. Coating material for cell
culture studies. ELISA
Reconstitution
Sterile pure water or PBS
Storage
Storage of lyophilized
product 2 years at -20°C. Storage of reconstituted product 1 month
at +4°C, 1 year at -20°C or lower. Avoid multiple freeze-thaw
cycles
Citations
1. Engelbertsen D, Rattik S, Knutsson A, Björkbacka H, Bengtsson E, Nilsson J. Induction of T helper 2 responses against human apolipoprotein B100 does not affect atherosclerosis in ApoE-/- mice. Cardiovasc Res. 2014 Jul 15;103(2):304-12. doi: 10.1093/cvr/cvu131. Epub 2014 May 27. PubMed PMID: 24866382
2. Negi P, Lövgren J, Malmi P, Sirkka N, Metso J, Huovinen T, Brockmann EC, Pettersson K, Jauhiainen M, Lamminmäki U. (2015) Identification and analysis of anti-HDL scFv-antibodies obtained from phage display based synthetic antibody library. Clin Biochem. 2016 Apr;49(6):472-479. doi: 10.1016/j.clinbiochem.2015.11.020. Epub 2015 Dec 2 PMID: 26656638
