Apolipoprotein B (ApoB) protein, a low-density lipoprotein (LDL) component, is purified from human plasma for use in research applications.
Apolipoprotein B is obtained from human plasma, with donors tested negative for HBsAg, HBc, HIV-I, HIV-II, and HCV antibodies.
Purity of at least 90%, as determined by SDS-PAGE, with a 550 kDa band.
The product is lyophilised from non-sterile solution containing 50 mM Na2CO3 (pH 10), 50 mM NaCl, and deoxycholate.
Purification involves ultracentrifugation in a density gradient, delipidation with diethyl ether and ethanol, and gel filtration on Sephacryl S-300.
Apolipoprotein B serves as a standard for apolipoprotein B research, an antigen for immunization, antibody production, and use in ELISA.
Reconstitute with sterile pure water or phosphate-buffered saline (PBS).
For reconstituted product, store for up to one month at +4°C or up to one year at -20°C or below. Avoid multiple freeze-thaw cycles.
1. Engelbertsen D, Rattik S, Knutsson A, Björkbacka H, Bengtsson E, Nilsson J. Induction of T helper 2 responses against human apolipoprotein B100 does not affect atherosclerosis in ApoE-/- mice. Cardiovasc Res. 2014 Jul 15;103(2):304-12. doi: 10.1093/cvr/cvu131. Epub 2014 May 27. PubMed PMID: 24866382