Bovine collagen type III, purified by partial pepsin digestion and differential salt precipitation, supplied lyophilised and salt-free at >90% purity, 10 mg. A fibrillar collagen of extensible connective tissue (skin, blood vessels, lung, intestine, uterus), co-distributed with collagen type I and a major constituent of reticular fibres. Use as an ELISA / Western blot standard, as immunogen, or as a cell-culture coating substrate — the bulk-format complement to the human antigen cat. 889, and the matching standard for the anti-collagen III antibodies cat. 153 and cat. 380.
SPECIFICATIONS
Catalogue number: 169 | Product: Collagen type III (bovine)
Source: bovine tissue
Purity: collagen type III >90%; other collagen types <10%
Purification: extraction into dilute acetic acid, followed by partial pepsin digestion under acidic conditions and differential salt precipitation. Pepsin treatment removes the non-helical telopeptide regions, reducing immunogenicity and improving solubility while retaining the intact triple-helical structure
Format: lyophilized, salt-free
Package size: 10 mg
STORAGE / RECONSTITUTION
Dissolve in 20 mM acetic acid. Shake or stir the suspension for several hours or overnight at 2–8 °C. The protein is fully dissolved when the solution appears homogeneous with no schlieren pattern (visible light refraction caused by differing protein concentrations).
Recommended working concentration: 1–5 mg/mL.
Lyophilized protein: ships at ambient temperature; store long-term at −20 °C or below (stable for 2 years).
Reconstituted protein: store at +4 °C for up to 1 month.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- Protein standard for collagen type III ELISA and Western blot — pair with cat. 153 or cat. 380 as the detection antibody.
- Immunogen for production of anti-collagen type III antibodies.
- Cell-culture coating substrate for adhesion, migration and proliferation assays.
- Substrate for matrix metalloproteinase (MMP) activity assays.
- Fibrosis and ECM remodelling research.
- Coating material for collagen-binding assay development.
APPLICATION HIGHLIGHTS
- Scaffold and biomaterial fabrication: supplied at 10 mg, the bulk format suited to crosslinked collagen scaffolds and blended type I/III matrices (see Reference).
- Wound healing and tissue remodelling: type III collagen is deposited early in granulation tissue and is a standard readout in repair models.
- Cardiovascular and pulmonary ECM: type III is abundant in vessel wall, lung and other extensible tissues.
- Telopeptide-depleted (atelo) form: reduced immunogenicity and improved solubility make it well suited to cell-culture coating and gel work.
- Defined type-III standard: >90% type III with <10% other collagen types, so it can serve as a calibrator where type specificity matters.
REFERENCES
Michopoulou et al. (2022) J. Biomed. Mater. Res. A. doi:10.1002/jbm.a.37329. Used as the type-III component (0.05 wt %, 20:1 I:III ratio) in EDC/NHS-crosslinked, heparin-functionalised collagen scaffolds.
