Bovine collagen type III, purified from bovine tissue by partial pepsin digestion and differential salt precipitation. Supplied lyophilised and salt-free at >90% purity. Suitable for use as an ELISA and Western blot standard, immunogen for antibody production, and coating substrate for cell culture and cell adhesion assays.
Background
Collagen type III is a fibrillar collagen found predominantly in extensible connective tissues including skin, blood vessels, lung, intestine and uterus. It is co-distributed with collagen type I in most tissues and is a major structural component of reticular fibres. Type III collagen plays an important role in wound healing, cardiovascular biology and fibrosis research, and is a clinically relevant biomarker in liver fibrosis and tissue remodelling studies.
This bovine collagen type III preparation is purified to >90% type III with less than 10% contaminating collagen types, making it suitable as a reference standard and immunogen where cross-reactivity between collagen types must be minimised.
Source and Purification
Collagen was extracted from dissected bovine tissue into dilute acetic acid. Type III collagen was isolated by partial pepsin digestion under acidic conditions followed by differential salt precipitation. Pepsin treatment removes the non-helical telopeptide regions, reducing immunogenicity and improving solubility while retaining the intact triple-helical collagen structure.
Specifications
- Source: Bovine tissue
- Purity: Collagen type III >90%; other collagen types <10%
- Format: Lyophilized, salt-free
- Purification: Partial pepsin digestion, differential salt precipitation
Applications
- Protein standard for collagen type III ELISA and Western blot
- Immunogen for production of anti-collagen type III antibodies
- Cell culture coating substrate for adhesion, migration and proliferation assays
- Substrate for matrix metalloproteinase (MMP) activity assays
- Fibrosis and ECM remodelling research
- Coating material for collagen-binding assay development
Reconstitution
Dissolve in 20 mM acetic acid. Shake or stir the suspension for several hours or overnight at 2–8°C. The protein is fully dissolved when the solution appears homogeneous with no schlieren pattern (visible light refraction caused by differing protein concentrations). Recommended working concentration: 1–5 mg/ml.
Storage
Lyophilized protein: ship at ambient temperature; store long-term at -20°C or below (stable for 2 years). Reconstituted protein: store at +4°C for up to 1 month. Avoid repeated freeze-thaw cycles of reconstituted material.
Citations
Michopoulou et al., J Biomed Mater Res A 2022, doi:10.1002/jbm.a.37329.
Used as the type-III component (0.05 wt %, 20:1 I:III ratio) in EDC/NHS-crosslinked, heparin-functionalised lyophilised collagen scaffolds. The resulting Coll I/III–Cross–Hep scaffolds reproduced the papillary-vs-reticular dermal heterogeneity in 3D culture of primary human dermal fibroblasts — increased swelling, papillary-fibroblast proliferation, and carbohydrate-rich ECM deposition — demonstrating the product as a low-immunogenicity calf-skin type III collagen suitable for dermal-equivalent and tissue-engineering scaffolds.
