Native bovine type I collagen extracted from calf skin by acetic-acid solubilisation following pepsin digestion, with type I isolation by differential salt precipitation. Supplied as a lyophilized powder. Composition is =90% bovine type I, non-collagen proteins below 0.5%, with the native triple-helical conformation preserved through purification — confirmed by the protein's ability to self-assemble into microfibrils on neutralisation.
For applications that require sterile filtration and a 3D-gel-tested formulation, the recommended upgrade is cat. 437 (bovine atelocollagen type I, sterile, lyophilized, 3D-gel grade). For a bovine type I + type III blend, see cat. 634. Cat. 631 itself sits in the YO Proteins type-I-collagen species map alongside the porcine (cat. 223, cat. 130, cat. 785, cat. 882), rat (cat. 351, cat. 602), goat (cat. 601), mouse (cat. 342) and human (cat. 288) type I SKUs.
SPECIFICATIONS
Catalogue number: 631
Amount: 10 mg
Source: Bovine (calf) skin
Purity: Bovine collagen type I =90%. Non-collagen proteins <0.5%. Native conformation confirmed by microfibril self-assembly
Format: Lyophilized
Purification: Partial pepsin digestion under acidic conditions; differential salt precipitation
STORAGE / RECONSTITUTION
Dissolve in 0.1 M acetic acid, pH 3.0. The dissolved collagen retains the immunological properties of native collagen and the ability to self-assemble into microfibrils on neutralisation.
Lyophilized protein: shipping at ambient temperature; long-term storage (2 years) at -20 °C or lower. Reconstituted protein: 1 month at +4 °C.
APPLICATIONS
- Bovine type I collagen reference standard for ELISA, immunoblot and dot-blot quantification of collagen synthesis, degradation, glycation (AGE) and turnover in cell, tissue and biofluid samples.
- Antigen for anti-bovine-collagen antibody production — pure species-matched immunogen for rabbit, mouse, goat, sheep or chicken immunisation programmes targeting anti-bovine type I collagen polyclonal or monoclonal antibodies.
- Culture-surface coating substrate for 2D cell culture — supports adhesion and proliferation of fibroblasts, osteoblasts, hepatocytes, smooth-muscle cells, mesenchymal stem cells, induced pluripotent stem cells, cancer-cell lines and many primary cell types where bovine type I is the historical default coating.
- Substrate for collagen-glycation studies — native bovine collagen I can be glycated in vitro (mannose, rhamnose, arabinose, glucose) to investigate AGE-driven matrix changes, AGE-receptor (RAGE) interactions and diabetic / aging matrix pharmacology.
- Substrate for type-I-specific protease-activity assays — defined bovine substrate for MMP-1, MMP-8, MMP-13, cathepsin K, fibroblast-activation-protein (FAP) and collagenase cleavage assays in fibrotic-disease, atherosclerosis and cancer-invasion drug discovery.
- Substrate for collagen-induced platelet-aggregation studies — the native collagen microfibrils that self-assemble on neutralisation activate platelets via the GPVI and a2ß1 integrin receptors, supporting platelet-activation, thrombosis and antiplatelet-drug-discovery research.
- Cross-species reactivity reference material — used alongside the porcine (cat. 223), rat (cat. 351), goat (cat. 601), mouse (cat. 342) and human (cat. 288) type I collagens in cross-species reactivity panels for anti-collagen antibody specificity validation.
- Substrate for fibrillogenesis and structural biophysics — defined bovine type I for in-vitro fibril self-assembly experiments and for AFM, optical-tweezers, persistence-length, SAXS / SANS and rheology characterisation work.
- Substrate for fibroblast pharmacology — bovine type I collagen quantification underpins screens of nicotinamide, copper, retinoids, antioxidants and cosmeceutical candidates in dermal-aging and wound-healing fibroblast cell models.
- Antigen for halal-authentication research — bovine is a halal-permissible animal source for collagen. cat. 631 is the bovine reference standard for halal-certified medical-device development, halal cosmetic-ingredient validation, and detection-of-porcine-contamination assays in halal authentication workflows.
- Reference material for collagen detection / quantification in food, cosmetic and biopharmaceutical industry research — defined bovine type I standard for cross-validation of in-house ELISA, HPLC, mass-spec and dot-blot assays.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Economical 10 mg bulk format for routine culture-surface coating and for preparing acid-soluble collagen gels.
- Native, self-assembling type I collagen for in vitro fibrillogenesis and microfibril-formation studies.
- Standard substrate for cell adhesion, spreading and migration assays across fibroblast, epithelial and tumour lines.
- Reference antigen and immunogen for anti-bovine / cross-reactive anti-collagen type I antibody work.
- Base material for collagen scaffolds, sponges and coated biomaterials in tissue-engineering and wound-healing models.
- Substrate for platelet-adhesion / aggregation and for collagen-receptor (integrin a1ß1, a2ß1, GPVI, DDR) binding studies.
