Native goat type I collagen extracted from caprine connective tissue by partial pepsin digestion under acidic conditions, followed by differential salt precipitation. Supplied as a lyophilized, salt-free powder. The composition is 90% type I and 10% type III, with other collagens at less than 1% combined and non-collagen proteins below 0.5%. On reconstitution in dilute acetic acid the collagen retains its native triple-helical conformation, with the immunological reactivity and microfibril self-assembly behaviour that define native collagen.
Goat type I collagen is rarely offered by the major research-reagent catalogues — most commercial type I collagens are bovine, porcine, rat or human. cat. 601 fills that gap with a defined-source caprine reagent for veterinary research, dairy and livestock biology, and any cross-species workflow where a species-matched goat standard is required. The goat origin also makes cat. 601 the appropriate type I collagen for halal-compliance research, including halal-certified medical-device development, halal cosmetic-ingredient validation, and detection-of-porcine-contamination assays where goat collagen is the reference halal-permissible animal-source standard.
cat. 601 is the caprine counterpart to the bovine (cat. 631, cat. 437), porcine (cat. 223, cat. 130, cat. 785, cat. 882), rat (cat. 351, cat. 602) and human (cat. 288) type I collagens in the YO Proteins range. It is also the appropriate companion reagent to the goat antibody-host SKUs across the YO catalogue when a researcher needs to verify cross-reactivity of a goat-host antibody back against its own species' collagen.
Specifications
Catalog number: 601
Amount: 10 mg
Source: Goat (caprine connective tissue)
Purity: Goat collagen type I 90%. Goat collagen type III 10%. Other collagens <1%. Non-collagen proteins <0.5%
Format: Lyophilized, salt-free
Purification: Partial pepsin digestion under acidic conditions; differential salt precipitation
Reconstitution
Dissolve in 0.5 M acetic acid, pH 2.5. The dissolved collagen retains the immunological properties of native collagen, and the native triple-helical structure is confirmed by the protein's ability to self-assemble into microfibrils on neutralisation.
Storage
Shipping at ambient temperature. Long-term storage: 2 years at -20 °C or lower.
Applications
- Goat type I collagen reference standard for ELISA, immunoblot and dot-blot quantification of collagen synthesis, degradation and turnover in caprine cell, tissue and biofluid samples.
- Antigen for anti-goat-collagen antibody production — pure species-matched immunogen for rabbit, mouse, sheep or chicken immunisation programmes targeting anti-goat type I collagen polyclonal or monoclonal antibodies.
- Culture-surface coating substrate for primary goat cells — caprine fibroblasts, MSCs, chondrocytes, hepatocytes, synoviocytes, mammary epithelial cells and stromal cells maintain a more native phenotype on species-matched collagen.
- Veterinary research substrate — defined caprine collagen for studies of caprine arthritis-encephalitis virus (CAEV) joint pathology, contagious caprine pleuropneumonia, foot rot, scrapie, paratuberculosis and other diseases of small ruminants.
- Dairy and livestock-biology research — substrate for caprine mammary epithelial culture, udder-tissue fibroblast studies, lactational biology and reproductive-tract cell models in dairy-goat research.
- Halal-compliance research — goat is a halal-permissible animal source for collagen. cat. 601 is the species-matched reference standard for halal-certified medical-device development, halal cosmetic-ingredient validation, halal pharmaceutical-excipient testing and detection-of-porcine-contamination assays in halal authentication workflows.
- Cross-species reactivity reference material — used alongside the bovine (cat. 631), porcine (cat. 223), rat (cat. 351), mouse and human (cat. 288) type I collagens in cross-species reactivity panels for anti-collagen antibody specificity validation.
- Substrate for type-I-specific protease-activity assays — defined goat substrate for MMP-1, MMP-8, MMP-13, cathepsin K and FAP-driven collagenase cleavage assays in caprine in-vitro work.
- Substrate for collagen-induced platelet-aggregation studies — the native collagen microfibrils that self-assemble on neutralisation activate platelets via the GPVI and a2ß1 integrin receptors, supporting caprine platelet-activation, thrombosis and antiplatelet-drug-discovery research relevant to ruminant haemostasis.
- Single-molecule biophysics — native triple-helical goat type I collagen for AFM, optical-tweezers and persistence-length structural studies of caprine collagen for comparative collagen biophysics.
- Antigen for anti-collagen autoantibody screening in caprine autoimmune-joint-disease research (CAE arthritis, idiopathic caprine polyarthritis).
