Mouse collagen type I, purified from mouse tail tendon by partial pepsin digestion under acidic conditions followed by differential salt precipitation, supplied lyophilised and salt-free, 1 mg. Pepsin treatment removes the non-helical telopeptide regions, lowering immunogenicity while preserving the intact triple-helical structure — native conformation is confirmed by the ability of the reconstituted protein to form microfibrils. Intended primarily as a species-matched murine collagen type I coating substrate and immunoassay standard; for 3D gel / hydrogel work use the sterile atelocollagen format cat. 603. It complements the murine collagen type I and III preparation cat. 221, and is the matching standard for the anti-collagen type I antibodies cat. 287 and cat. 393.
SPECIFICATIONS
Catalogue number: 342 | Product: Collagen type I (mouse)
Source: mouse tail tendon
Purity: murine collagen type I – 90%; collagen type III – 10%; other collagen types below 1%; non-collagen proteins below 0.5%
Purification: partial pepsin digestion under acidic conditions, followed by differential salt precipitation
Format: lyophilized, salt-free
Package size: 1 mg
STORAGE / RECONSTITUTION
Dissolve in 0.5 M acetic acid, pH 2.5, at +4 °C. Collagen solubility: 10 mg/mL.
Dissolved collagen retains the immunological properties of native collagen; the native structure is confirmed by its ability to form microfibrils.
Lyophilized protein: ships at ambient temperature; store long-term at −20 °C or below (stable for 2 years).
Reconstituted protein: store at +4 °C for up to 1 month.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- Coating material for cell-culture studies — 2D surface coating for adhesion, migration and proliferation assays. Note: this preparation may not be suitable for 3D gel formation; for 3D collagen gels use the sterile murine atelocollagen cat. 603.
- Species-matched murine protein standard for collagen type I ELISA and Western blot — pair with cat. 287 or cat. 393 as the detection antibody.
- Immunogen for production of anti-collagen type I antibodies.
- Substrate for matrix metalloproteinase (MMP) activity assays.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Murine-model concordance: a species-matched matrix for in vitro assays that support mouse in vivo studies, removing xenogeneic collagen as a confounding variable.
- Fibrosis and ECM remodelling research in murine disease models.
- Collagen-receptor signalling studies (integrin – and DDR-mediated) on defined 2D matrices.
- Platelet and immune-cell adhesion assays on murine collagen surfaces.
- Stem-cell and organoid substrate optimisation where donor species matching is critical.
