Native rat collagen type I, extracted from rat tail tendon by partial pepsin digestion under acidic conditions followed by differential salt precipitation, supplied lyophilised and salt-free, 20 mg. On reconstitution in dilute acetic acid the collagen retains its native triple-helical conformation, with the immunological reactivity and microfibril self-assembly behaviour that define native collagen. Cat. 351 is the rat-source counterpart to the bovine (cat. 631, cat. 437), porcine (cat. 223) and human (cat. 288) type I collagens in the range, and the appropriate choice whenever species-matched rat collagen is required — anti-rat-collagen immunisation programmes, rodent ELISA and dot-blot standards, coating of culture plates for rat primary cells, and collagen-induced platelet-aggregation studies. Pair with cat. 376 (anti-rat collagen I) or cat. 352 (anti-rat collagen I + III) for detection; for 3D gel work use the rat atelocollagen cat. 602.
SPECIFICATIONS
Catalogue number: 351 | Product: Collagen type I (rat)
Source: rat tail tendon
Purity: rat collagen type I 90%; rat collagen type III 10%; other collagens <1% combined; non-collagen proteins <0.5%
Purification: partial pepsin digestion under acidic conditions; differential salt precipitation
Format: lyophilised, salt-free | Package size: 20 mg
STORAGE / RECONSTITUTION
Dissolve in 0.5 M acetic acid, pH 2.5. The dissolved collagen retains the immunological properties of native collagen; the native triple-helical structure is confirmed by self-assembly into microfibrils on neutralisation.
Lyophilised protein: ships at ambient temperature; store long-term at −20 °C or lower (stable for 2 years).
Reconstituted protein: store at +4 °C for up to 4 weeks, or aliquot and freeze.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- Rat collagen type I reference standard for ELISA, immunoblot and dot-blot quantification of collagen synthesis, degradation and turnover in rodent cell, tissue and biofluid samples — pair with cat. 376 or cat. 352.
- Species-matched immunogen for anti-rat collagen antibody production (rabbit, mouse, goat or chicken immunisation programmes).
- Culture-surface coating substrate for rat primary cells (cardiomyocytes, hepatocytes, neurons, fibroblasts, MSCs, smooth-muscle cells).
- Collagen-induced platelet-aggregation studies: microfibrils self-assembled on neutralisation activate platelets through GPVI and integrin a2b1.
- Substrate for type-I-specific protease assays (MMP-1, MMP-8, MMP-13, cathepsin K, FAP).
- Cross-species reference material alongside the human (cat. 288), porcine (cat. 223) and bovine (cat. 631) type I collagens for antibody-specificity panels.
- Single-molecule biophysics: native triple-helical collagen for AFM, optical-tweezers and persistence-length studies.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Rodent-to-large-animal translational studies: the rodent-source ECM control in cell-migration and wound-healing assays run head-to-head with porcine collagen.
- Thrombosis and antiplatelet drug discovery: native fibrillar collagen as the physiological platelet agonist.
- Rat primary-cell culture where a species-matched matrix avoids cross-species adhesion artefacts.
- Bulk 20 mg format for coating-intensive workflows and immunisation programmes.
- Fibrosis and ECM-turnover research in rat disease models.
REFERENCES
O’Brien K., Bhatia A., Tsen F., Chen M., Wong A.K., et al. (2014) Identification of the critical therapeutic entity in secreted Hsp90α that promotes wound healing in newly re-standardised healthy and diabetic pig models. PLoS ONE 9(12): e113956. doi:10.1371/journal.pone.0113956. PMID 25464502.
