Rat collagen type I
Source
Rat tail tendon. Collagen
was extracted from washed dissected tissue into dilute acetic acid
after pepsin treatment. Collagen type I was purified by using
differential salt precipitation
Purity
Rat collagen type I 90%.
Rat collagen type III 10%. Other collagens below 1%. Non-collagen
proteins below 0.5%
Format
Lyophilized, salt-free
Purification
Partial pepsin digestion
in acidic conditions and differential salt precipitation
Applications
Rat collagen (type
I) standard. Antigen for antibody
production. Coating material for cell culture studies. Collagen
microfibrils will activate platelets causing aggregation. May not be suitable for 3-D gel formation
Reconstitution
Use 0.5 M acetic acid, pH
2.5. Dissolved collagen retains immunologic properties of native
collagen. Structure of native collagen confirmed by ability to form
microfibrils
Storage
Shipping at ambient
temperature. Storage 2 years at -20°C or lower
Citations
1. O’Brien K, Bhatia A, Tsen F, Chen M, Wong AK, et al. (2014) Identification of the Critical Therapeutic Entity in Secreted Hsp90a That Promotes Wound Healing in Newly ReStandardized Healthy and Diabetic Pig Models. PLoS ONE 9(12): e113956. doi:10.1371/ journal.pone.0113956 (use in pig cell migration assay) PMID: 25464502
