Canine collagen type I and III, sourced from canine tissue and purified using differential salt precipitation, serves as an excellent coating material for cell culture studies.
Source
Collagen is extracted from washed, dissected canine tissue using dilute acetic acid following pepsin treatment. Collagen type I and III are purified via differential salt precipitation.
Purity
The product contains 98% canine collagen type I and III, with less than 1% of other canine collagen types and less than 0.5% non-collagen proteins.
Format
The collagen is provided in a lyophilized, salt-free format.
Purification
Purification involves partial pepsin digestion in acidic conditions followed by differential salt precipitation.
Applications
This canine collagen is ideal as a coating material for cell culture studies. However, it may not be suitable for 3-D gel formation.
Reconstitution
To reconstitute the collagen, use 0.5 M acetic acid (pH 2.5). Mix vigorously overnight, and employ a homogenizer if needed to disperse any remaining sediment.
Storage
The lyophilized protein can be shipped at ambient temperature and stored for 2 years at -20°C. Reconstituted protein can be stored for 1 month at +4°C.
Publications
Ferkau A, Gillmann HJ, Mischke R, Calmer S, Ecklebe S, Abid M, Minde JW, Echtermeyer F, Theilmeier G. (2013) Infection-associated platelet dysfunction of canine platelets detected in a flow chamber model. BMC Vet Res. 2013 Jun 7;9(1):112 (PMID: 23758817)
Ferkau A, Ecklebe S, Jahn K, Calmer S, Theilmeier G, Mischke R. (2013) A dynamic flow-chamber-based adhesion assay to assess canine platelet-matrix interactions in vitro. Vet Clin Pathol. 2013 Jun;42(2):150-6 (PMID: 23627964)
