Canine collagen type I and III

Source

Canine tissue. Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment. Collagen type I and III was purified by using differential salt precipitation

Purity

Canine collagen I and III – 98%, canine collagen (other types) <1%, non-collagen proteins <0.5%

Format

Lyophilized, salt-free

Purification

Partial pepsin digestion in acidic conditions and differential salt precipitation

Applications

Coating material for cell culture studies. May not be suitable for 3-D gel formation

Reconstitution

Use 0.5 M acetic acid, pH 2.5. Mix vigorously overnight and use homogenizer if required to disperse the recalcitrant sediment. Dissolved collagen retains immunologic properties of native collagen. Structure of native collagen confirmed by ability to form microfibrils

Storage

Shipping at ambient temperature. Storage of lyophilized protein 2 years at -20°C. Storage of reconstituted protein 1 month at +4°C

Publications

Ferkau A, Gillmann HJ, Mischke R, Calmer S, Ecklebe S, Abid M, Minde JW, Echtermeyer F, Theilmeier G. (2013) Infection-associated platelet dysfunction of canine platelets detected in a flow chamber model. BMC Vet Res. 2013 Jun 7;9(1):112 (PMID: 23758817)

Ferkau A, Ecklebe S, Jahn K, Calmer S, Theilmeier G, Mischke R. (2013) A dynamic flow-chamber-based adhesion assay to assess canine platelet-matrix interactions in vitro. Vet Clin Pathol. 2013 Jun;42(2):150-6 (PMID: 23627964)