Canine collagen
type I and III
Source
Canine tissue. Collagen
was extracted from washed dissected tissue into dilute acetic acid
after pepsin treatment. Collagen type I and III was purified by using
differential salt precipitation
Purity
Canine collagen I and III
– 98%, canine collagen (other types) <1%, non-collagen proteins
<0.5%
Format
Lyophilized, salt-free
Purification
Partial pepsin digestion
in acidic conditions and differential salt precipitation
Applications
Coating material for cell
culture studies. May not be suitable for 3-D gel formation
Reconstitution
Use 0.5 M acetic acid, pH
2.5. Mix vigorously overnight and use homogenizer if required to
disperse the recalcitrant sediment. Dissolved collagen retains
immunologic properties of native collagen. Structure of native
collagen confirmed by ability to form microfibrils
Storage
Shipping at ambient
temperature. Storage of lyophilized protein 2 years at -20°C.
Storage of reconstituted protein 1 month at +4°C
Publications
Ferkau A, Gillmann HJ, Mischke R, Calmer S, Ecklebe S, Abid M, Minde JW, Echtermeyer F, Theilmeier G. (2013) Infection-associated platelet dysfunction of canine platelets detected in a flow chamber model. BMC Vet Res. 2013 Jun 7;9(1):112 (PMID: 23758817)
Ferkau A, Ecklebe S, Jahn K, Calmer S, Theilmeier G, Mischke R. (2013) A dynamic flow-chamber-based adhesion assay to assess canine platelet-matrix interactions in vitro. Vet Clin Pathol. 2013 Jun;42(2):150-6 (PMID: 23627964)
