Porcine collagen type I and III purified from porcine tendons
Source
Porcine tendons. Collagen
was extracted from washed dissected tissue into dilute acetic acid
after pepsin treatment. Collagen type I and III was purified by using
differential salt precipitation
Purity
Porcine collagens: type I
and III – 95%, other collagen types below 1%.
Non-collagen proteins below 0.5%
Format
Lyophilized, salt-free
Purification
Partial pepsin digestion
in acidic conditions and differential salt precipitation
Applications
Standard for immunological assays. Coating material for cell
culture studies. May not be suitable for 3-D gel formation
Pig cell migration assay (1)
Reconstitution
Use acetic acid, pH
2.5 in concentration from 0.02 to 0.5M. Dissolved collagen retains immunologic properties of native
collagen. Structure of native collagen confirmed by ability to form
microfibrils
Storage
Shipping at ambient
temperature. Storage of lyophilized protein at -20°C.
Storage of reconstituted protein 1 month at +4°C
Citations
1. O’Brien K, Bhatia A,
Tsen F, Chen M, Wong AK, et al. (2014) Identification of the Critical
Therapeutic Entity in Secreted Hsp90a That Promotes Wound Healing in
Newly ReStandardized Healthy and Diabetic Pig Models. PLoS ONE 9(12):
e113956. doi:10.1371/ journal.pone.0113956 PMID: 25464502
2. Yang Zhang, Yi Chen, Bo Zhao, Jianping Gao, Leilei Xia, Fangyu Xing, Yingjun Kong, Yongchao Li and Guifeng Zhang. Detection of Type I and III collagen in porcine acellular matrix using HPLC–MS. Regenerative Biomaterials, 2020, 1–6
