Native-conformation porcine collagen types I and III, co-purified from porcine Achilles tendon by partial pepsin digestion under acidic conditions followed by differential salt precipitation, supplied lyophilised and salt-free, 10 mg. Reconstituted material retains the immunological reactivity of native collagen and forms microfibrils, confirming preservation of the triple-helical structure. Intended as a soluble reference, coating substrate and immunoassay antigen rather than as a 3D-gel precursor — for 3D gel work use the porcine atelocollagen cat. 785. Pair with the anti-porcine collagen I + III antibody cat. 386 for immunoassay detection. Related porcine collagen formats: cat. 882 (native collagen I), cat. 130 (sterile atelocollagen I) and the collagen bioink cat. 144.
SPECIFICATIONS
Catalogue number: 223 | Product: Collagen type I and III (porcine)
Source: porcine Achilles tendon
Purity: collagen types I and III combined >95%; other collagen types <1%; non-collagen proteins <0.5%
Purification: partial pepsin digestion under acidic conditions; differential salt precipitation
Format: lyophilised powder, salt-free | Package size: 10 mg
Not suitable for: 3D collagen gel formation.
STORAGE / RECONSTITUTION
Dissolve in 0.02–0.5 M acetic acid, pH 2.5. Do not vortex and do not heat.
Lyophilised protein: ships at ambient temperature; store long-term at −20 °C or lower (stable for 2 years).
Reconstituted protein: store at +4 °C for up to 4 weeks, or aliquot and freeze.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- ELISA and immunoblot standard for porcine collagen types I and III — pair with cat. 386 as the detection antibody.
- Immunogen for raising anti-porcine collagen antibodies (rabbit / mouse immunisation protocols).
- HPLC–MS calibration standard for marker-peptide quantification of collagen types I and III in tissue digests and decellularised biomaterials.
- Species-matched coating substrate for porcine cell culture and 2D cell-migration assays.
- Reference material for acellular-matrix (ACM) and decellularised-tissue research requiring type-specific collagen quantification.
- Substrate in porcine wound-healing models, including colloidal-gold migration and re-epithelialisation assays.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Decellularised-scaffold QC: the calibrator of choice for quantifying residual collagen I / III in porcine SIS, dermis and pericardium.
- Species matching for porcine models: avoids the cross-species artefacts seen when rat- or bovine-source collagen is substituted.
- Xenograft and biomaterial regulatory characterisation of porcine-derived implant matrices.
- Mass-spectrometry method development: defined type-specific marker peptides for targeted MRM assays.
- Fibrosis research: the type I : type III ratio as a marker of matrix maturation.
REFERENCES
Zhang Y., et al. (2020) Quantification of collagen in porcine acellular matrix by HPLC–MS with marker peptides. Regenerative Biomaterials 7(6): 577–582. doi:10.1093/rb/rbaa032.
