Discover Human Collagen Type IV: A Versatile Biomaterial for Advanced Research
Unlock the potential of human collagen type IV, a vital component of the extracellular matrix, with our high-quality purified product derived from human placenta. Ensuring exceptional purity, our collagen type IV is 95% pure, with other human collagens below 5% and non-collagen proteins below 0.5%. Our lyophilized, salt-free format undergoes partial pepsin digestion in acidic conditions and differential salt precipitation for optimal purification.
This versatile biomaterial is ideal for a range of applications, including immunological assays, antibody production, and cell culture studies. As a standard for ELISA and dot blotting (1), human collagen type IV has been utilized to polarize THP1 monocyte differentiation (2) and enhance cell attachment in conjugated ECM-PA microarrays (3). Additionally, it has been employed for the fabrication of cell microarrays to investigate drug response in lung adenocarcinoma cells (4) and to rescue dying siNotch3 ovarian cancer cells (5).
Human adipose-derived stem cells (ASCs) have also been successfully cultured on human collagen type IV (7), showcasing its diverse applicability. Reconstitution is simple, using 0.5 M acetic acid, pH 2.5. Shipped at ambient temperature, our product offers a 2-year shelf life when stored at -20°C or lower.
Citations
1. Kuzan A, Chwilkowska A, Maksymowicz K, Szydelko-Bronowicka A, Stach K, Pezowicz C, Gamian A (2018) Advanced glycation end products as a source of artifacts in immunoenzymatic methods. Glycoconjugate Journal 35(1) DOI: 10.1007/s10719-017-9805-4
2. Pal S, V. Badireenath Konkimalla (2016) Data on sulforaphane treatment mediated suppression of autoreactive, inflammatory M1 macrophages. Data in Brief. https://doi.org/10.1016/j.dib.2016.03.105
3. Kourouklis AP, Kaylan KB, Underhill GH (2016) Substrate stiffness and matrix composition coordinately control the differentiation of liver progenitor cells. Biomaterials 99:82-94
4. Kaylan KB, Gentile SD, Milling LE, Bhinge KN, Kosari F and Underhill GH (2016) Mapping lung tumor cell drug responses as a function of matrix context and genotype using cell microarrays. Integrative Biology. Issue 12
5. Brown CW, Brodsky AS and Freiman RN (2015) Notch3 Overexpression Promotes Anoikis Resistance in Epithelial Ovarian Cancer via Upregulation of COL4A2. Mol. Cancer Res. vol 13, issue 1
6. Kaylan KB, Kourouklis AP, Underhill GH A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces. https://www.jove.com/video/55362
7. Clevenger TN, Hinman CR, Ashley Rubin RK, Smither K, Burke DJ, Hawker CJ, Messina D, van Epps D, Clegg DO (2016) Vitronectin-Based, Biomimetic Encapsulating Hydrogel Scaffolds Support Adipogenesis of Adipose Stem Cells. Tissue Engineering Part A. 22:7-8
