Human Alpha-Fetoprotein (AFP) for Research and Diagnostic Assay Development
Alpha-fetoprotein (AFP) is a 70 kDa serum glycoprotein and the principal plasma protein of the human fetus. It is widely used as a clinical biomarker for hepatocellular carcinoma (HCC), non-seminomatous germ cell tumours and prenatal screening for neural tube defects and Down syndrome. This preparation is purified from human cord blood and is suitable for use as a calibrator or reference standard in AFP ELISA kits, immunoassay development and Western blot applications.
Source
Human cord blood. Negative for HBsAg, HIV and HCV.
Purification
Hydrophobic interaction, ion-exchange and affinity chromatography, sulphate fractionation and MonoQ-FPLC.
Purity
95% by SDS-PAGE — single band at 70 kDa at a load of 25 mg/lane.
Format
Lyophilized.
Applications
- ELISA calibrator and reference standard for AFP immunoassays
- Antigen for anti-AFP antibody production and validation
- Western blot and immunoblotting standard
- Tumor marker assay development (hepatocellular carcinoma, testicular cancer)
- Prenatal screening assay development
- IVD kit manufacturing and quality control
Reconstitution
Reconstitute in sterile PBS pH 7.4. Mix gently — do not vortex. Allow to dissolve at 2–8°C. Recommended working concentration depends on application; refer to your assay protocol for appropriate dilution range.
Storage
Lyophilized protein: ship at ambient temperature; store at -20°C or below for up to 2 years. Reconstituted protein: store at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Quantity pricing available — order 2 or more units at a reduced rate. Contact us for bulk pricing on larger quantities.
Citations
1. Jee JE, Ang YL, Cha J, Ang MW, Ling J, Lim J, Lee SS. Combinatorial bead-based peptide libraries improved for rapid and robust screenings. Comb Chem High Throughput Screen. 2014;17(6):520-30. PubMed PMID: 24410000
