Human integrin α1β1 (VLA-1; CD49a/CD29; ITGA1/ITGB1), affinity-purified from human smooth muscle and supplied lyophilized, 5 µg. A collagen-binding β1 integrin: in ELISA it interacts with collagen type I, collagen type IV and laminin. Supplied in a format specified by the manufacturer as appropriate for functional evaluation — use with cat. 288 (human collagen type I) and cat. 425 as immobilised ligands in receptor–ligand binding work.
SPECIFICATIONS
Catalogue number: 126 | Product: Integrin α1β1 (human)
Also known as: VLA-1, CD49a/CD29, ITGA1
Source: human smooth muscle. Product was tested and found negative for HIV and Hepatitis B
Purity: ≥ 95% by SDS-PAGE with silver staining. Two major bands corresponding to the α1 (195 kDa) and β1 (120 kDa) subunits under non-reducing conditions; a minor band (210 kDa) corresponds to the β1 subunit disulphide dimer
Purification: affinity chromatography. The tissue detergent extract applied to the column was prepared as described in Belkin et al. (1990)
Format: lyophilized from a solution containing 0.28 mg/mL α1β1, 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.2% Triton X-100
Package size: 5 µg
STORAGE / RECONSTITUTION
Reconstitute in water.
Lyophilized product ships at ambient temperature; for long-term storage keep at −20 °C.
Reconstituted protein: store at −70 °C, where it is stable for 1 year.
Avoid repeated freeze-thaw cycles.
APPLICATIONS
- ELISA receptor–ligand binding: α1β1 binds immobilised collagen type I (cat. 288), collagen type IV (cat. 425) and laminin.
- Functional evaluation of integrin–ligand interaction; the detergent-containing format is supplied for functional work.
- Ligand-competition and inhibitor-screening formats using collagen or laminin as the immobilised ligand.
- Solid-phase binding assays and surface coating for adhesion-receptor studies.
APPLICATION HIGHLIGHTS
- Collagen-receptor selectivity: α1β1 is one of the collagen-binding integrins, useful for dissecting collagen type I versus collagen type IV recognition alongside the matching YO Proteins antigens.
- Basement-membrane adhesion: both collagen type IV and laminin are basement-membrane constituents and are bound by this receptor in ELISA.
- Purified heterodimer: the preparation retains both α1 and β1 chains (confirmed by SDS-PAGE), so receptor stoichiometry is defined.
- Native-source material: purified from human tissue rather than recombinantly expressed, for studies where post-translational context matters.
REFERENCES
Belkin et al. (1990) J. Cell Biol. 111:2159–2170.
