Human integrin a1b1,
affinity purified, lyophilized (VLA-1, CD49a/CD29, ITGA1)
Source
Human smooth muscles.
Product was tested and found negative for HIV and Hepatitis B
Purity
SDS-PAGE
with silver staining at least 95%. Two major bands corresponding to
a1 (195 kDa) and b1
(120 kDa) subunits (non-reduced conditions). A minor band (210 kDa)
corresponds to b1 subunit
disulphide dimer.
Format
Lyophilized from solution
containing: 0.28 mg/ml a1b1, 20 mM
Tris-HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.2% Triton
X-100
Purification
Affinity
chromatography. A tissue detergent extract, which was applied to the
column was prepared as described in (Belkin et al. 1990, J. Cell.
Biol. 111:2159-2170)
Applications
Integrin a1b1
interacts with collagen I, IV and laminin in ELISA. This format of
a1b1 is appropriate for functional
evaluation.
Reconstitution
Water. Keep reconstituted
protein at -70°C. Avoid
repeated freeze-thaw cycles
Storage
Lyophilized
product: shipping at ambient temperature, long-term storage at -20°C.
Reconstituted protein: 1 year at -70°C
