Human integrin α5β1 (fibronectin receptor, VLA-5; ITGA5 / ITGB1, alias CD49e, FNRA), affinity-purified from human placenta and supplied lyophilised, 5 µg. The purified heterodimer runs as two major bands under non-reducing conditions, corresponding to the α5 (155 kDa) and β1 (110 kDa) subunits. α5β1 is the classical RGD-dependent receptor for fibronectin, governing cell adhesion, fibronectin fibrillogenesis, migration and survival signalling. Supplied as a defined standard for electrophoresis, immunoblotting and ligand-binding studies; it is the companion product to the vitronectin receptor αVβ5 cat. 476, binds the fibronectin heparin-binding fragment cat. 815, and is complemented by the anti-fibronectin antibody cat. 770.
SPECIFICATIONS
Catalogue number: 638 | Product: Integrin α5β1 (human)
Source: human placenta. The product was tested and found negative for HIV and Hepatitis B
Gene: ITGA5 / ITGB1 | Aliases: VLA-5, CD49e, FNRA, alpha5beta1
Purity: at least 95% by SDS-PAGE with silver staining; two major bands corresponding to the α5 (155 kDa) and β1 (110 kDa) subunits under non-reducing conditions
Purification: affinity chromatography; the tissue detergent extract applied to the column was prepared as described in Belkin et al. 1990
Format: lyophilized from a solution containing 0.26 mg/mL α5β1, 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.2% Triton X-100
Package size: 5 µg
STORAGE / RECONSTITUTION
Reconstitute in water.
Lyophilized product: ships at ambient temperature; store long-term at −20 °C.
Reconstituted protein: store at −70 °C (stable for 1 year).
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- Molecular-weight and identity standard for SDS-PAGE / electrophoresis of integrin heterodimers.
- Positive control and standard for immunoblotting (Western blot) of the α5 and β1 integrin subunits.
- Solid-phase ligand-binding assays with fibronectin and RGD-containing peptides — pair with the fibronectin 40 kDa heparin-binding fragment cat. 815.
- Coating antigen for integrin ELISA and antibody-characterisation work.
- Immunogen and screening antigen for the generation of anti-integrin antibodies.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- RGD-mimetic and integrin-antagonist drug discovery: purified receptor for competitive ligand-binding and inhibitor IC50 screening.
- Fibronectin fibrillogenesis and matrix-assembly research, where α5β1 is the principal assembly receptor.
- Tumour progression and angiogenesis studies: α5β1 is upregulated on angiogenic endothelium and invasive carcinoma.
- Mechanotransduction and adhesion-force research (the α5β1–fibronectin catch-bond is a canonical model system).
- Stem-cell adhesion and pluripotency-maintenance studies on defined fibronectin substrates.
- Biomaterial and RGD-peptide surface characterisation for regenerative-medicine scaffolds.
REFERENCES
Belkin VM, Belkin AM, Koteliansky VE. Human smooth muscle VLA-1 integrin: purification, substrate specificity, localization in aorta, and expression during development. J Cell Biol. 1990;111(5 Pt 1):2159–2170. doi:10.1083/jcb.111.5.2159. PMID: 2172263. (Source of the tissue detergent-extract preparation used for affinity purification.)
