Human integrin a5b1,
affinity purified, lyophilized (VLA-5, ITGA5, CD49e, VLA5A, FNRA, alpha5beta1)
Source
Human placenta. Product
was tested and found negative for HIV and Hepatitis B
Purity
SDS-PAGE
with silver staining at least 95%. Two major bands corresponding to
a5 (155 kDa) and b1
(110 kDa) subunits (non-reduced conditions).
Format
Lyophilized from solution
containing: 0.26 mg/ml a5b1, 20 mM
Tris-HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.2% Triton
X-100
Purification
Affinity
chromatography. A tissue detergent extract, which was applied to the
column was prepared as described in (Belkin et al. 1990, J. Cell.
Biol. 111:2159-2170)
Applications
Standard for
electrophoresis, immunoblotting and ligand binding
Reconstitution
Water. Keep reconstituted
protein at -70?C. Avoid
repeated freeze-thaw cycles
Storage
Lyophilized
product: shipping at ambient temperature, long-term storage at -20?C.
Reconstituted protein: 1 year at -70?C
