Human integrin aVb3, affinity purified, lyophilized. Also known as vitronectin receptor, vitronectin integrin, CD51, CD61, ITGAV, ITGB3, VNRA, alphaVbeta3)
Source
Human placenta. Product
was tested and found negative for HIV and Hepatitis B
Purity
SDS-PAGE
with silver staining at least 95%. Two major bands corresponding to
aV (145 kDa) and b3
(92 kDa) subunits (non-reduced conditions). A minor band (170 kDa)
corresponds to b3 subunit
disulphide dimer.
Format
Lyophilized from solution
containing: 0.21 mg/ml aVb3,
20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.2%
Triton X-100
Purification
Affinity
chromatography with immobilized monoclonal anti-aVb3
antibodies. A tissue detergent extract, which was applied to the
column was prepared as described in (Belkin et al. 1990, J. Cell.
Biol. 111:2159-2170)
Applications
Integrin aVb3
interacts with vitronectin, von Willebrand factor, fibronectin and
fibrinogen in RGD dependent ELISA test. This format of aVb3
is appropriate for functional evaluation.
- Standard for
electrophoresis, immunobloting and ligand binding
- In vitro binding
experiments with metalloproteinase MMP-2
Reconstitution
Water. Keep reconstituted
protein at -70°C. Avoid
repeated freeze-thaw cycles
Storage
Lyophilized
product: shipping at ambient temperature, long-term storage at -20°C.
Reconstituted protein: 1 year at -70°C
Reference
Wirkner M, Weis S, San Miguel V, Álvarez M, Gropeanu RA, Salierno M, Sartoris A, Unger RE, Kirkpatrick CJ, del Campo A. (2011) Photoactivatable caged cyclic RGD peptide for triggering integrin binding and cell adhesion to surfaces. Chembiochem. 12(17):2623-9
Zhang B, Song W, Pang P, Zhao Y, Zhang P, Csabai I, Vattay G and Lindsay S (2017) Observation of giant conductance fluctuations in a protein. Nano Futures, Volume 1, Number 3
Gc, J.B., Chen, J., Pokharel, S.M. et al. Molecular basis for the recognition of 24-(S)-hydroxycholesterol by integrin avß3. Sci Rep 13, 9166 (2023). https://doi.org/10.1038/s41598-023-36040-4
