Human integrin aVb5,
affinity purified, lyophilized (ITGAV, MSK8, CD51, VNRA, alphaVbeta5)
Source
Human placenta. Product
was tested and found negative for HIV and Hepatitis B
Purity
SDS-PAGE
with silver staining at least 95%. Two major bands corresponding to
aV (145 kDa) and b5
(90 kDa) subunits (non-reduced conditions).
Format
Lyophilized from solution
containing: 0.26 mg/ml aVb5,
20 mM Tris-HCl pH 7.5, 150 mM NaCl, 2 mM MgCl2, 0.2%
Triton X-100
Purification
Affinity
chromatography with immobilized monoclonal anti-aVb5
antibodies. A tissue detergent extract, which was applied to the
column was prepared as described in (Belkin et al. 1990, J. Cell.
Biol. 111:2159-2170)
Applications
Standard for
electrophoresis, immunoblotting and ligand binding
Reconstitution
Water. Keep reconstituted
protein at -70°C. Avoid
repeated freeze-thaw cycles
Storage
Lyophilized
product: shipping at ambient temperature, long-term storage at -20°C.
Reconstituted protein: 1 year at -70°C
