Murine collagen types I and III co-purified from mouse tail tendons. Supplied lyophilized and salt-free for use as an immunoassay standard, cell culture coating substrate and antigen for anti-mouse collagen antibody production. Not recommended for 3D gel formation.
Source: Mouse tail tendons
Purity
- Collagen type I: 45%
- Collagen type III: 45%
- Collagen type IV: 10%
- Collagen type V: less than 1%
- Non-collagen proteins: less than 0.5%
Format: Lyophilized, salt-free
Purification: Partial pepsin digestion, differential salt precipitation
Applications
- Murine collagen type I/III ELISA and Western blot standard
- Anti-mouse collagen antibody production and cross-reactivity testing
- Cell culture coating for fibroblast and connective tissue assays
- Species-matched ECM substrate for murine cell culture models
Reconstitution: Dissolve in 0.05–0.5 M acetic acid pH 2.5 at 4°C.
Retains immunological properties of native collagen. Capable of microfibril formation. Do not vortex or heat.
Storage: Lyophilized — ambient shipping, 2 years at -20°C.
Reconstituted — 1 month at +4°C.
