Murine collagen types I and III, co-purified from mouse tail tendons by partial pepsin digestion and differential salt precipitation, supplied lyophilised and salt-free, 5 mg. The material retains the immunological properties of native collagen and is capable of microfibril formation, but is not recommended for 3D gel formation — for gel work use the mouse atelocollagen cat. 603. Use as a species-matched immunoassay standard, as a cell-culture coating substrate, and as immunogen for anti-mouse collagen antibody production. Complementary reagents: single-type mouse collagen I cat. 342 and cat. 176; the rodent counterpart cat. 362 (rat collagen I + III); and the cross-reactive antibodies cat. 352 and cat. 478.
SPECIFICATIONS
Catalogue number: 221 | Product: Collagen type I and III (mouse)
Source: mouse tail tendons
Purity: collagen type I 45%; collagen type III 45%; collagen type IV 10%; collagen type V <1%; non-collagen proteins <0.5%
Purification: partial pepsin digestion; differential salt precipitation
Format: lyophilised, salt-free | Package size: 5 mg
STORAGE / RECONSTITUTION
Dissolve in 0.05–0.5 M acetic acid, pH 2.5, at +4 °C. Do not vortex and do not heat.
Lyophilised protein: ships at ambient temperature; store long-term at −20 °C or lower (stable for 2 years).
Reconstituted protein: store at +4 °C for up to 4 weeks, or aliquot and freeze.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- Murine collagen type I / III ELISA and Western blot standard — pair with cat. 352 or cat. 478 as the detection antibody.
- Immunogen for anti-mouse collagen antibody production and for cross-reactivity testing.
- Cell-culture coating substrate for fibroblast and connective-tissue adhesion, migration and proliferation assays.
- Species-matched ECM substrate for murine cell-culture models.
- Substrate for matrix metalloproteinase (MMP) activity assays on murine collagen.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Species matching: avoids the cross-species epitope artefacts that arise when murine cells or murine sera are assayed against bovine or human collagen.
- Fibrosis and wound-healing models: the type I / type III ratio is the classic readout of scar maturation, and this preparation carries both types together.
- Antibody development: native-conformation murine immunogen for antibodies intended for use on mouse tissue.
- Tumour-microenvironment and ECM-remodelling studies in murine xenograft and syngeneic models.
- Microfibril assembly studies — the preparation retains fibril-forming competence, unlike fully atelo preparations.
