Mouse monoclonal antibody against human IgM (µ heavy chain), clone ICL-931, unconjugated, 0.5 mL. Recognises a single defined epitope on the human IgM µ chain, so every lot delivers identical reactivity — the reproducible alternative to polyclonal anti-IgM reagents such as the goat cat. 321, rabbit cat. 818 and sheep cat. 898. The flexible unconjugated format for ELISA capture, Western primary, flow cytometry (after in-house conjugation), immunoprecipitation, or as a competitor antibody to map patient anti-IgM autoantibody epitopes.
SPECIFICATIONS
Catalogue number: 445 | Host: Mouse | Target: Human
Target antigen: Human IgM, µ heavy chain (defined epitope)
Gene: IGHM (14q32.33) | UniProt: P01871
Clonality: Monoclonal | Clone: ICL-931 | Conjugate: Unconjugated
Specificity: Human IgM (µ heavy chain); binds one defined epitope, giving lot-to-lot-consistent reactivity.
Format: Liquid, unconjugated | Package size: 0.5 mL
STORAGE
Ships at ambient temperature. Store at +4 °C for short-term use; for long-term storage aliquot and freeze at -20 °C or lower. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — capture antibody in IgM-specific sandwich ELISA where lot-to-lot reproducibility is critical.
- Western blot — primary antibody for human IgM heavy chain.
- Flow cytometry — human IgM+ B-cell subsets (after fluorophore conjugation).
- Immunoprecipitation / co-IP — single-epitope binding gives clean elution of human IgM complexes.
- Epitope mapping — competition assays against patient anti-IgM autoantibodies.
- Custom in-house conjugation — to biotin, AP or a fluorophore.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Serology & infectious disease — isotype-specific IgM detection of acute-phase antibody responses.
- Autoimmunity — IgM autoantibody and rheumatoid-factor assays.
- B-cell immunology — identification of IgM+ naive and memory B-cell subsets.
- Reference & QC reagent — defined-epitope binding for antibody-panel cross-validation.
- Assay development — reproducible capture geometry for standardised diagnostic ELISAs.
REFERENCES
Notz Q, Schmalzing M, Wedekink F, et al. (2020) Pro- and anti-inflammatory responses in severe COVID-19-induced acute respiratory distress syndrome — an observational pilot study. Frontiers in Immunology 11:581338. doi:10.3389/fimmu.2020.581338.
