Native porcine collagen type I, purified from porcine tendon by extraction into dilute acetic acid and differential salt precipitation, supplied sterile, lyophilised and salt-free, 25 mg. Extracted without pepsin, so the non-helical telopeptide regions are retained — the native, telopeptide-intact form that self-assembles into strong fibrillar 3-D gels on neutralisation. Use as a cell-culture coating substrate and 3-D gel matrix — the native counterpart to the telopeptide-depleted porcine atelocollagen preparations cat. 785 and cat. 130, and detectable with the anti-porcine collagen type I + III antibody cat. 386.
SPECIFICATIONS
Catalogue number: 882 | Product: Collagen type I (porcine, native)
Source: porcine tendons
Purity: porcine collagen type I >90%; porcine collagen type III <10%
Sterility: passed sterility testing for bacteria and fungi
Format: lyophilised, salt-free; sterile
Purification: extraction from washed dissected tissue into dilute acetic acid, followed by differential salt precipitation. No pepsin treatment — telopeptides are retained (native collagen)
Package size: 25 mg
STORAGE / RECONSTITUTION
Reconstitution: dissolve in 20 mM acetic acid at a collagen concentration of 5 mg/mL. Shake or stir the suspension for several hours or overnight at 2–8 °C. The material is dissolved when it appears homogeneous, with no schlieren pattern (light refraction caused by differing protein concentrations in the suspension). Recommended working concentration 1–5 mg/mL.
Shipped at ambient temperature.
Lyophilised protein: long-term storage 2 years at −20 °C or lower.
Reconstituted protein: store at +4 °C for up to 1 month.
Avoid repeated freeze-thaw cycles of reconstituted material.
APPLICATIONS
- Cell-culture coating substrate for adhesion, migration and proliferation assays.
- 3-D gel formation for three-dimensional culture, organoid, spheroid and contraction assays.
- Protein standard for collagen type I ELISA and Western blot — pair with cat. 386 as the detection antibody.
- Immunogen for production of anti-collagen type I antibodies.
- Substrate for matrix metalloproteinase (MMP) activity and collagen-binding assays.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Native, telopeptide-intact collagen: retaining the telopeptides preserves the natural crosslinking sites and gives firmer, more physiological fibrillar gels than atelocollagen — the format of choice where fibril architecture and gel mechanics matter.
- Fibril self-assembly and mechanobiology: suitable for studies of fibrillogenesis, gel contraction, matrix stiffness and traction-force work.
- Sterile and salt-free: can be taken straight into cell culture without dialysis or further clean-up.
- Porcine source for translational models: closely similar to human collagen and widely used where a non-bovine, non-human matrix is required.
- Tendon-derived type I: the tissue in which type I collagen is most abundant and most highly organised, giving a well-defined starting material.
- Bulk 25 mg format: sufficient for large gel volumes, scaffold casting and repeated coating campaigns.
