Rabbit affinity-purified polyclonal antibody against rat collagen type III, 1 mL. Cross-absorbed against rat collagen type I, with only minimal cross-reactivity to type I — so it reads type-III specifically within the rat fibrillar-collagen pair. The type-III counterpart to cat. 376 (rabbit anti-rat collagen I); run the two together for side-by-side type-I-vs-III fibril mapping, or step up to the pan-fibrillar cat. 352 (rabbit anti-rat collagen I+III) for a single readout. Optimised for native-conformation work: rat collagen-III sandwich ELISA, frozen-section histochemistry, and immunostaining of collagen-III ECM in cell culture.
SPECIFICATIONS
Catalogue number: 864 | Host: Rabbit | Target: Rat
Target antigen: Rat collagen type III
Gene: COL3A1
Clonality: Polyclonal | Conjugate: Unconjugated
Specificity: Binds rat collagen type III; minimal cross-reactivity to rat collagen type I; cross-absorbed against rat collagen type I. Native-conformation — not suitable for denaturing or dissociating conditions.
Purification: Affinity-purified on immobilised rat collagen type III, cross-absorbed on immobilised rat collagen type I
Format: Lyophilised from 0.01 M sodium phosphate, 0.01 M sodium borate, 0.15 M sodium chloride, 1% dextran, 1% mannitol, pH 8 | Package size: 1 mL
STORAGE / RECONSTITUTION
Reconstitute with 1 mL sterile pure water. Ships at ambient temperature. Store lyophilised antibody 2 years at -20 °C or lower. After reconstitution, aliquot and store frozen; avoid repeated freeze-thaw.
APPLICATIONS
- ELISA — detection of rat collagen type III by sandwich ELISA (dilution x1000).
- IHC / histochemistry (frozen sections) — rat collagen-III histochemistry (dilution x100).
- Immunocytochemistry / ECM staining — immunostaining of collagen-III extracellular matrix in cell culture.
- Type-III-vs-I discrimination — cross-absorbed against rat collagen I; pair with cat. 376 (type I) to resolve the two interstitial fibrillar collagens.
- Note: native-conformation antibody — not suitable for denatured Western blot, FFPE (HIER), or other dissociating conditions.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Rat tendon, ligament & wound healing — collagen-III deposition in early repair and remodelling (e.g. patellar tendon healing).
- Rat fibrosis models — type-III collagen in renal, hepatic, cardiac, pulmonary and dermal fibrosis.
- Vascular & visceral ECM — collagen-III mapping in rat vascular and interstitial tissue.
- Scar maturation — early type-III collagen and its transition toward type-I in rat repair models.
- Collagen-scaffold tissue engineering — localisation / QC of collagen-III in rat scaffold and regenerative studies.
REFERENCES
Chan BP, Fu S-c, Qin L, Lee K-m, Rolf CG, Chan K-m. (2000) Effects of basic fibroblast growth factor (bFGF) on early stages of tendon healing: a rat patellar tendon model. Acta Orthop Scand 71(5): 513–518. PMID: 11186411.
