Specificity
Binds rat collagen
type III. Minimal cross-reactivity to rat collagen type I
Preparation
Affinity purification on
immobilized rat collagen type III with cross-absorption with rat
collagen type I
Format
Lyophilized from 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1%mannitol, pH 8
Applications and
recommended dilutions
Detection of rat collagen
type III by ELISA (x1000), Histochemistry (frozen sections)
(x100)(1). Immunostaining of ECM in cell culture. Not suitable for
denaturing and dissociating conditions.
Reconstitution
Use 1 ml sterile pure
water
Storage
Shipping at ambient
temperature. Storage of lyophilized antibodies 2 years at -20°C or
lower
Citations
1) Chan BP, Fu S-c, Qin L, Lee K-m, Rolf CG, Chan K-m (2000) Effects of basic fibroblast growth factor (bFGF) on early stages of tendon healing. A rat patellar tendon model. Acta Orthop Scand 2000; 71 (5): 513–518 PMID: 11186411