Purified mouse immunoglobulin G (IgG), 1 mg — whole-molecule IgG isolated from normal mouse serum. Supplied as an immunochemical reference material: ELISA standard and calibrator for total mouse-IgG quantification, isotype / irrelevant-antibody control in immunostaining and flow cytometry, coating antigen for secondary-antibody titration, immunogen for raising anti-mouse IgG antisera, and blocking / adsorption reagent. Pairs with the anti-mouse IgG antibody panel — goat anti-mouse IgG + IgM + IgA cat. 133 and rabbit anti-mouse IgG + IgM + IgA cat. 696, and belongs to the purified immunoglobulin panel alongside human IgG cat. 697, rat IgG cat. 147, mouse IgG cat. 883, rabbit IgG cat. 700, bovine IgG cat. 404 and human IgA cat. 258.
SPECIFICATIONS
Catalogue number: 883 | Source: Normal mouse serum
Composition: Immunoglobulin G (IgG), whole molecule
Format: Purified immunoglobulin | Package size: 1 mg
STORAGE / RECONSTITUTION
Ships at ambient temperature. Store at −20 °C or lower for long-term storage. After reconstitution or thawing store at +4 °C for short-term use, or aliquot and freeze. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA standard / calibrator for total mouse-IgG quantification in serum, plasma and culture supernatant.
- Isotype and irrelevant-antibody control for mouse antibodies in IHC, ICC and flow cytometry.
- Coating antigen and positive control for titration and QC of anti-mouse IgG secondary conjugates — see the anti-mouse IgG antibody panel — goat anti-mouse IgG + IgM + IgA cat. 133 and rabbit anti-mouse IgG + IgM + IgA cat. 696.
- Immunogen for raising anti-mouse IgG antisera and secondary conjugates.
- Blocking / adsorption reagent to remove anti-mouse reactivity from multi-species assay systems.
- Reference protein for chromatography method development (Protein A / Protein G binding, IgG capture) — see also Protein A cat. 339.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Hybridoma and monoclonal antibody development — isotype control and standard-curve material.
- Murine immunology — total-IgG quantification in infection, vaccination and knockout models.
- IHC / flow cytometry — irrelevant-antibody control matched to mouse monoclonal primaries.
- Mouse-on-mouse staining — blocking and adsorption reagent to suppress background.
- Chromatography development — mouse IgG subclass binding behaviour on Protein A (cat. 339).
