Purified human immunoglobulin G (IgG), 1 mg — whole-molecule IgG isolated from normal human serum. Supplied as an immunochemical reference material: ELISA standard and calibrator for total human-IgG quantification, isotype / irrelevant-antibody control in immunostaining and flow cytometry, coating antigen for secondary-antibody titration, immunogen for raising anti-human IgG antisera, and blocking / adsorption reagent. Pairs with the anti-human IgG antibody panel — goat anti-human IgG cat. 454, rabbit anti-human IgG cat. 493 and mouse anti-human IgG clone EFE-565 cat. 534, and belongs to the purified immunoglobulin panel alongside human IgG cat. 697, rat IgG cat. 147, mouse IgG cat. 883, rabbit IgG cat. 700, bovine IgG cat. 404 and human IgA cat. 258.
SPECIFICATIONS
Catalogue number: 697 | Source: Normal human serum
Composition: Immunoglobulin G (IgG), whole molecule
Format: Purified immunoglobulin | Package size: 1 mg
STORAGE / RECONSTITUTION
Ships at ambient temperature. Store at −20 °C or lower for long-term storage. After reconstitution or thawing store at +4 °C for short-term use, or aliquot and freeze. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA standard / calibrator for total human-IgG quantification in serum, plasma and culture supernatant.
- Isotype and irrelevant-antibody control for human antibodies in IHC, ICC and flow cytometry.
- Coating antigen and positive control for titration and QC of anti-human IgG secondary conjugates — see the anti-human IgG antibody panel — goat anti-human IgG cat. 454, rabbit anti-human IgG cat. 493 and mouse anti-human IgG clone EFE-565 cat. 534.
- Immunogen for raising anti-human IgG antisera and secondary conjugates.
- Blocking / adsorption reagent to remove anti-human reactivity from multi-species assay systems.
- Reference protein for chromatography method development (Protein A / Protein G binding, IgG capture) — see also Protein A cat. 339.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Clinical immunology research — calibration of total-IgG and IgG-subclass immunoassays.
- Immunodeficiency and hypergammaglobulinaemia studies — reference material for quantitative serology.
- Therapeutic antibody and IVIG research — Protein A / Protein G binding and purification method development.
- Autoimmune serology — isotype control and blocking reagent in anti-human IgG detection systems.
- Multi-species immunoassay design — cross-reactivity testing and adsorption of anti-human reactivity.
- Fc-receptor and complement-activation studies — purified human IgG as a defined binding ligand.
