Purified Protein A, 1 mg — the cell-wall protein of Staphylococcus aureus that binds with high affinity to the Fc region of immunoglobulin G from many mammalian species (strong binding to human, rabbit and guinea-pig IgG; weaker binding to mouse, rat and bovine IgG). Supplied as a purified reagent for IgG capture and purification, immunoprecipitation, and Fc-mediated detection. Works directly with the purified immunoglobulin panel — human IgG cat. 697, rat IgG cat. 147, mouse IgG cat. 883, rabbit IgG cat. 700, bovine IgG cat. 404 and human IgA cat. 258 — which provides matched binding and negative-control material for method development.
SPECIFICATIONS
Catalogue number: 339 | Source: Staphylococcus aureus
Composition: Protein A, Fc-binding protein
Format: Purified protein | Package size: 1 mg
STORAGE / RECONSTITUTION
Ships at ambient temperature. Store at −20 °C or lower for long-term storage. After reconstitution or thawing store at +4 °C for short-term use, or aliquot and freeze. Avoid repeated freeze-thaw.
APPLICATIONS
- Affinity capture and purification of IgG from serum, ascites, hybridoma supernatant and culture medium.
- Immunoprecipitation and co-immunoprecipitation — Fc-mediated capture of antibody-antigen complexes.
- Coupling to solid supports, beads and resins for antibody chromatography method development.
- Fc-binding detection reagent in ELISA, dot-blot and Western blot formats (Protein A conjugates).
- Species cross-reactivity and binding-strength profiling using the matched purified IgG panel — cat. 697, 700, 883, 147 and 404; human IgA cat. 258 serves as a non-binding control.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Antibody manufacturing and downstream process development — resin screening and dynamic binding capacity studies.
- Hybridoma and monoclonal antibody workflows — small-scale IgG purification from supernatant.
- Immunoprecipitation-based interactomics — pull-down of antibody-bound complexes for MS analysis.
- Biosensor and SPR assay development — Fc-capture surfaces for antibody kinetics.
- Staphylococcal biology and immune-evasion research — Protein A as a B-cell superantigen and virulence factor.
