Purified rabbit immunoglobulin G (IgG), 1 mg — whole-molecule IgG isolated from normal rabbit serum. Supplied as an immunochemical reference material: ELISA standard and calibrator for total rabbit-IgG quantification, isotype / irrelevant-antibody control in immunostaining and flow cytometry, coating antigen for secondary-antibody titration, immunogen for raising anti-rabbit IgG antisera, and blocking / adsorption reagent. Pairs with the anti-rabbit IgG antibody panel — goat anti-rabbit IgG + IgM + IgA cat. 333 and sheep anti-rabbit IgG + IgM + IgA cat. 596, and belongs to the purified immunoglobulin panel alongside human IgG cat. 697, rat IgG cat. 147, mouse IgG cat. 883, rabbit IgG cat. 700, bovine IgG cat. 404 and human IgA cat. 258.
SPECIFICATIONS
Catalogue number: 700 | Source: Normal rabbit serum
Composition: Immunoglobulin G (IgG), whole molecule
Format: Purified immunoglobulin | Package size: 1 mg
STORAGE / RECONSTITUTION
Ships at ambient temperature. Store at −20 °C or lower for long-term storage. After reconstitution or thawing store at +4 °C for short-term use, or aliquot and freeze. Avoid repeated freeze-thaw.
APPLICATIONS
- ELISA standard / calibrator for total rabbit-IgG quantification in serum, plasma and culture supernatant.
- Isotype and irrelevant-antibody control for rabbit antibodies in IHC, ICC and flow cytometry.
- Coating antigen and positive control for titration and QC of anti-rabbit IgG secondary conjugates — see the anti-rabbit IgG antibody panel — goat anti-rabbit IgG + IgM + IgA cat. 333 and sheep anti-rabbit IgG + IgM + IgA cat. 596.
- Immunogen for raising anti-rabbit IgG antisera and secondary conjugates.
- Blocking / adsorption reagent to remove anti-rabbit reactivity from multi-species assay systems.
- Reference protein for chromatography method development (Protein A / Protein G binding, IgG capture) — see also Protein A cat. 339.
APPLICATION HIGHLIGHTS — HIGH-VALUE RESEARCH NICHES
- Secondary-antibody QC — the single most-used control antigen for anti-rabbit conjugate titration.
- Polyclonal antibody development — pre-immune / irrelevant rabbit IgG control for immunisation studies.
- IHC and ICC — negative and isotype control matched to rabbit primary antibodies.
- Chromatography method development — rabbit IgG binds Protein A strongly, an ideal model analyte (cat. 339).
- Immunoassay blocking — adsorption of anti-rabbit reactivity in multiplex systems.
